Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model

BMC Ophthalmology

Research Article

Laura Raimondi¹  Jean-Jacques Gicquel²  Carlotta Boccalini³  Eleonora Favuzza³  Rita Mencucci³ 

[1] Department of NEUROFARBA, section of Pharmacology, University of Florence, Florence, Italy;Department of Ophthalmology, Poitiers University Hospital, Poitiers, Cedex, France;Department of Surgery and Translational Medicine – Eye Clinic, University of Florence, Florence, Italy;

关键词: Posterior capsule opacification;    Intraocular lens;    Square edge;    Cataract;   

DOI  :  10.1186/1471-2415-15-5

 received in 2014-07-24, accepted in 2015-01-10,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundTo evaluate lens epithelial cell (LEC) proliferation with two different designs (one-piece or three-piece) of hydrophobic acrylic IOLs with 360° square optic edge using an in vitro culture model of posterior capsule opacification (PCO).MethodsThis experimental study was conducted at the Department of NEUROFARBA, Section of Pharmacology, University of Florence, Italy. Human LECs were seeded and cultured in transwell cell culture inserts coated with a type-IV collagen membrane on which an IOL (one-piece Tecnis-1 or three-piece AR40E, Abbott Medical Optics Inc.) had been previously placed. As control, cells were plated on the insert membrane without an IOL. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated.ResultsNo statistically significant difference in the number of cells (p > 0.05) between inserts with the one-piece and three-piece IOLs was found. Cell density in the area under each IOL was significantly lower than in the area outside of it (p < 0.05), or in the control insert. (p < 0.05). Cell density under the single-piece IOL was not significantly different from that under the three-piece IOL (p > 0.05).ConclusionsA 360° sharp-edge played a crucial role in avoiding LEC migration under the IOL and preventing the formation of PCO after cataract surgery. Long term clinical evaluation is necessary to estimate functional results.

【 授权许可】

CC BY   
© Mencucci et al.; licensee BioMed Central. 2015