Transcriptional profiling of Arabidopsis root hairs and pollen defines an apical cell growth signature

BMC Plant Biology

Research Article

Liam Dolan¹  Seiji Takeda²  Jörg D Becker³  José A Feijó⁴  Filipe Borges⁵ 

[1] Department of Cell and Developmental Biology, John Innes Centre, NR4 7UH, Norwich, UK;Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, Oxford, UK;Department of Cell and Developmental Biology, John Innes Centre, NR4 7UH, Norwich, UK;Present address: Cell and Genome Biology, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kitaina-Yazuma Oji 74, Seika-cho, 619-0244, Soraku-gun, Kyoto, Japan;Instituto Gulbenkian de Ciência, 2780-156, Oeiras, Portugal;Instituto Gulbenkian de Ciência, 2780-156, Oeiras, Portugal;Department of Cell Biology and Molecular Genetics, University of Maryland, 0118 BioScience Research Bldg, 20742-5815, College Park, MD, USA;Instituto Gulbenkian de Ciência, 2780-156, Oeiras, Portugal;Present address: Cold Spring Harbor Laboratory, 11724, Cold Spring Harbor, NY, USA;

关键词: Pollen;    Pollen tube;    Root hair;    Transcriptome;    Apical growth;    Tip growth;    Apical signature;    Arabidopsis;   

DOI  :  10.1186/s12870-014-0197-3

 received in 2014-03-11, accepted in 2014-07-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCurrent views on the control of cell development are anchored on the notion that phenotypes are defined by networks of transcriptional activity. The large amounts of information brought about by transcriptomics should allow the definition of these networks through the analysis of cell-specific transcriptional signatures. Here we test this principle by applying an analogue to comparative anatomy at the cellular level, searching for conserved transcriptional signatures, or conserved small gene-regulatory networks (GRNs) on root hairs (RH) and pollen tubes (PT), two filamentous apical growing cells that are a striking example of conservation of structure and function in plants.ResultsWe developed a new method for isolation of growing and mature root hair cells, analysed their transcriptome by microarray analysis, and further compared it with pollen and other single cell transcriptomics data. Principal component analysis shows a statistical relation between the datasets of RHs and PTs which is suggestive of a common transcriptional profile pattern for the apical growing cells in a plant, with overlapping profiles and clear similarities at the level of small GTPases, vesicle-mediated transport and various specific metabolic responses. Furthermore, cis-regulatory element analysis of co-regulated genes between RHs and PTs revealed conserved binding sequences that are likely required for the expression of genes comprising the apical signature. This included a significant occurrence of motifs associated to a defined transcriptional response upon anaerobiosis.ConclusionsOur results suggest that maintaining apical growth mechanisms synchronized with energy yielding might require a combinatorial network of transcriptional regulation. We propose that this study should constitute the foundation for further genetic and physiological dissection of the mechanisms underlying apical growth of plant cells.

【 授权许可】

CC BY   
© Becker et al. 2014