Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress

BMC Genomics

Research Article

Richard J Giannone¹  Robert L Hettich¹  Nancy L Engle²  Lezlee Dice²  Timothy J Tschaplinski²  Zamin K Yang²  Steven D Brown²  Shihui Yang³ 

[1] BioEnergy Science Center, Oak Ridge National Laboratory, 1 Bethel Valley Road, 37831, Oak Ridge, Tennessee, USA;Chemical Sciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, 37831, Oak Ridge, Tennessee, USA;Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, 37831, Oak Ridge, Tennessee, USA;BioEnergy Science Center, Oak Ridge National Laboratory, 1 Bethel Valley Road, 37831, Oak Ridge, Tennessee, USA;Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Road, 37831, Oak Ridge, Tennessee, USA;BioEnergy Science Center, Oak Ridge National Laboratory, 1 Bethel Valley Road, 37831, Oak Ridge, Tennessee, USA;National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, 80401, Golden, Colorado, USA;

关键词: Cellobiose;    Glutamine Synthetase;    Ethanol Treatment;    Ethanol Tolerance;    Ethanol Stress;   

DOI  :  10.1186/1471-2164-13-336

 received in 2011-12-02, accepted in 2012-07-01,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundClostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol. However, C. thermocellum is relatively sensitive to ethanol compared to ethanologenic microorganisms such as yeast and Zymomonas mobilis that are used in industrial fermentations but do not possess native enzymes for industrial cellulose hydrolysis.ResultsIn this study, C. thermocellum was grown to mid-exponential phase and then treated with ethanol to a final concentration of 3.9 g/L to investigate its physiological and regulatory responses to ethanol stress. Samples were taken pre-shock and 2, 12, 30, 60, 120, and 240 min post-shock, and from untreated control fermentations for systems biology analyses. Cell growth was arrested by ethanol supplementation with intracellular accumulation of carbon sources such as cellobiose, and sugar phosphates, including fructose-6-phosphate and glucose-6-phosphate. The largest response of C. thermocellum to ethanol shock treatment was in genes and proteins related to nitrogen uptake and metabolism, which is likely important for redirecting the cells physiology to overcome inhibition and allow growth to resume.ConclusionThis study suggests possible avenues for metabolic engineering and provides comprehensive, integrated systems biology datasets that will be useful for future metabolic modeling and strain development endeavors.

【 授权许可】

Unknown   
© Yang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.