Transcriptome analysis of secondary cell wall development in Medicago truncatula

BMC Genomics

Research Article

Jung Hyun Yang¹  Huanzhong Wang¹  Qian Du¹  Yuhong Tang²  Jiangqi Wen²  Ivone Torres-Jerez²  Xiaofei Cheng²  Mingyi Wang²  Fang Chen³  Richard Dixon³ 

[1] Department of Plant Science and Landscape Architecture, University of Connecticut, 1390 Storrs Rd., 06269, Storrs, CT, USA;Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA;Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, 73401, Ardmore, OK, USA;Department of Biological Sciences, University of North Texas, 1155 Union Circle, 76203, Denton, TX, USA;

关键词: Transcriptome;    Expression;    Secondary cell wall;    Development;    Medicago truncatula;   

DOI  :  10.1186/s12864-015-2330-6

 received in 2015-04-29, accepted in 2015-12-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLegumes are important to humans by providing food, feed and raw materials for industrial utilizations. Some legumes, such as alfalfa, are potential bioenergy crops due to their high biomass productivity. Global transcriptional profiling has been successfully used to identify genes and regulatory pathways in secondary cell wall thickening in Arabidopsis, but such transcriptome data is lacking in legumes.ResultsA systematic microarray assay and high through-put real time PCR analysis of secondary cell wall development were performed along stem maturation in Medicago truncatula. More than 11,000 genes were differentially expressed during stem maturation, and were categorized into 10 expression clusters. Among these, 279 transcription factor genes were correlated with lignin/cellulose biosynthesis, therefore representing putative regulators of secondary wall development. The b-ZIP, NAC, WRKY, C2H2 zinc finger (ZF), homeobox, and HSF gene families were over-represented. Gene co-expression network analysis was employed to identify transcription factors that may regulate the biosynthesis of lignin, cellulose and hemicellulose. As a complementary approach to microarray, real-time PCR analysis was used to characterize the expression of 1,045 transcription factors in the stem samples, and 64 of these were upregulated more than 5-fold during stem maturation. Reverse genetics characterization of a cellulose synthase gene in cluster 10 confirmed its function in xylem development.ConclusionsThis study provides a useful transcriptome and expression resource for understanding cell wall development, which is pivotal to enhance biomass production in legumes.

【 授权许可】

CC BY   
© Wang et al. 2015