Inter- and intra-reproducibility of genotypes from sheep technical replicates on Illumina and Affymetrix platforms

Genetics Selection Evolution

Short Communication

Noirin McHugh¹  Donagh P. Berry¹  Aine O’Brien¹  Jenny Grose²  Stephen Park³  Shane Randles⁴  Kevin McDermott⁴  Eamonn Wall⁴  Rebecca Weld⁵  Paul Flynn⁵ 

[1] Animal and Grassland Research and Innovation Centre, Moorepark, Teagasc, Fermoy, Co. Cork, Ireland;GeneSeek, A Neogen Company, Lincoln, NE, USA;Identigen Ltd, Dublin, Ireland;Sheep Ireland, Highfield House, Shinagh, Bandon, Co. Cork, Ireland;Weatherbys Ltd, Naas, Ireland;

关键词: Call Rate;    Concordance Rate;    Illumina Platform;    Single Nucleotide Polymorphism Chip;    Affymetrix Platform;   

DOI  :  10.1186/s12711-016-0267-0

 received in 2016-08-12, accepted in 2016-11-04,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAccurate genomic analyses are predicated upon access to accurate genotype input data. The objective of this study was to quantify the reproducibility of genotype data that are generated from the same genotype platform and from different genotyping platforms.MethodsGenotypes based on 51,121 single nucleotide polymorphisms (SNPs) for 84 animals that were each genotyped on Illumina and Affymetrix platforms and for another 25 animals that were each genotyped twice on the same Illumina platform were compared. Genotypes based on 11,323 SNPs for an additional 21 animals that were genotyped on two different Illumina platforms by two different service providers were also compared. Reproducibility of the results was measured as the correlation between allele counts and as genotype and allele concordance rates.ResultsA mean within-animal correlation of 0.9996 was found between allele counts in the 25 duplicate samples that were genotyped on the same Illumina platform and varied from 0.9963 to 1.0000 per animal. The mean (minimum, maximum) genotype and allele concordance rates per animal between the 25 duplicate samples were equal to 0.9996 (0.9968, 1.0000) and 0.9993 (0.9937, 1.0000), respectively. The concordance rate between the two different Illumina platforms was also near 1. A mean within-animal correlation of 0.9738 was found between genotypes that were generated on the Illumina and Affymetrix platforms and varied from 0.9505 to 0.9812 per animal. The mean (minimum, maximum) within-animal genotype and allele concordance rates between the Illumina and Affymetrix platforms were equal to 0.9711 (0.9418, 0.9798) and 0.9845 (0.9695, 0.9889), respectively. The genotype concordance rate across all genotypes increased from 0.9711 to 0.9949 when the SNPs used were restricted to those with three high-resolution genotype clusters which represented 75.2% of the called genotypes.Conclusions and implicationsOur results suggest that, regardless of the genotype platform or service provider, high genotype concordance rates are achieved especially if they are restricted to high-quality extracted DNA and SNPs that result in high-quality genotypes.

【 授权许可】

CC BY   
© The Author(s) 2016