Transcriptome analysis of the whitefly, Bemisia tabaci MEAM1 during feeding on tomato infected with the crinivirus, Tomato chlorosis virus, identifies a temporal shift in gene expression and differential regulation of novel orphan genes

BMC Genomics

Research Article

Yi Zheng¹  Wenbo Chen¹  Zhangjun Fei¹  Navneet Kaur²  William M. Wintermantel²  Daniel K. Hasegawa³  Kai-Shu Ling³ 

[1] Boyce Thompson Institute, 533 Tower Road, 14853-1801, Ithaca, NY, USA;USDA-ARS, Crop Improvement and Protection Research, 1636 East Alisal Street, 93905, Salinas, CA, USA;USDA-ARS, U.S. Vegetable Laboratory, Charleston, 2700 Savannah Highway, 29414, Charleston, SC, USA;

关键词: Whitefly;    Bemisia tabaci;    Tomato chlorosis virus;    Crinivirus;    RNA-Seq;    Semipersistent transmission;    Orphan genes;    Gene expression;   

DOI  :  10.1186/s12864-017-3751-1

 received in 2017-01-06, accepted in 2017-05-02,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWhiteflies threaten agricultural crop production worldwide, are polyphagous in nature, and transmit hundreds of plant viruses. Little is known how whitefly gene expression is altered due to feeding on plants infected with a semipersistently transmitted virus. Tomato chlorosis virus (ToCV; genus Crinivirus, family Closteroviridae) is transmitted by the whitefly (Bemisia tabaci) in a semipersistent manner and infects several globally important agricultural and ornamental crops, including tomato.ResultsTo determine changes in global gene regulation in whiteflies after feeding on tomato plants infected with a crinivirus (ToCV), comparative transcriptomic analysis was performed using RNA-Seq on whitefly (Bemisia tabaci MEAM1) populations after 24, 48, and 72 h acquisition access periods on either ToCV-infected or uninfected tomatoes. Significant differences in gene expression were detected between whiteflies fed on ToCV-infected tomato and those fed on uninfected tomato among the three feeding time periods: 447 up-regulated and 542 down-regulated at 24 h, 4 up-regulated and 7 down-regulated at 48 h, and 50 up-regulated and 160 down-regulated at 72 h. Analysis revealed differential regulation of genes associated with metabolic pathways, signal transduction, transport and catabolism, receptors, glucose transporters, α-glucosidases, and the uric acid pathway in whiteflies fed on ToCV-infected tomatoes, as well as an abundance of differentially regulated novel orphan genes. Results demonstrate for the first time, a specific and temporally regulated response by the whitefly to feeding on a host plant infected with a semipersistently transmitted virus, and advance the understanding of the whitefly vector-virus interactions that facilitate virus transmission.ConclusionWhitefly transmission of semipersistent viruses is believed to require specific interactions between the virus and its vector that allow binding of virus particles to factors within whitefly mouthparts. Results provide a broader understanding of the potential mechanism of crinivirus transmission by whitefly, aid in discerning genes or loci in whitefly that influence virus interactions or transmission, and subsequently facilitate development of novel, genetics-based control methods against whitefly and whitefly-transmitted viruses.

【 授权许可】

CC BY   
© The Author(s). 2017