Dynamic parent-of-origin effects on small interfering RNA expression in the developing maize endosperm

BMC Plant Biology

Research Article

Chuang Ma¹  Ruolin Yang¹  Zhongfu Ni²  Xiangfeng Wang²  Qixin Sun²  Huiru Peng²  Mingming Xin²  Yingyin Yao² 

[1] School of Plant Sciences, University of Arizona, 1145 E. South Campus Drive, 85721-0036, Tucson, AZ, USA;State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, 100193, Beijing, China;

关键词: siRNA;    Imprinting;    Maize;    Endosperm;    GSE52726;   

DOI  :  10.1186/s12870-014-0192-8

 received in 2014-05-08, accepted in 2014-07-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundIn angiosperms, the endosperm plays a crucial placenta-like role in that not only is it necessary for nurturing the embryo, but also regulating embryogenesis through complicated genetic and epigenetic interactions with other seed compartments and is the primary tissue in which genomic imprinting occurs.ResultsWe observed a gradual increase of paternal siRNA expression in the early stages of kernels and an expected 2:1 maternal to paternal ratio in 7-DAP endosperm via sequencing of small interfering RNA (siRNA) transcriptomes in developing kernels (0, 3 and 5 days after pollination (DAP)) and endosperms (7, 10 and 15 DAP) from the maize B73 and Mo17 reciprocal crosses. Additionally, 460 imprinted siRNA loci were identified in the endosperm, with the majority (456/460, 99.1%) being maternally expressed at 10 DAP. Moreover, 13 out of 29 imprinted genes harbored imprinted siRNA loci within their 2-kb flanking regions, a significant higher frequency than expected based on simulation analysis. Additionally, gene ontology terms of “response to auxin stimulus”, “response to brassinosteroid stimulus” and “regulation of gene expression” were enriched with genes harboring 10-DAP specific siRNAs, whereas those of “nutrient reservoir activity”, “protein localization to vacuole” and “secondary metabolite biosynthetic process” were enriched with genes harboring 15-DAP specific siRNAs.ConclusionsA subset of siRNAs subjected to imprinted expression pattern in maize developing endosperm, and they are likely correlated with certain imprinted gene expression. Additionally, siRNAs might influence nutrient uptake and allocation processes during maize endosperm development.

【 授权许可】

Unknown   
© Xin et al. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.