Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) and cultivated V. radiata through genotyping by sequencing and quantitative trait locus analysis

BMC Plant Biology

Research Article

Chen-yu Lin¹  Shu-mei Huang¹  Roland Schafleitner¹  Shui-hui Chu¹  Chien-yu Chen²  Dung-chi Wu²  Chih-wei Tung³  Thu-Giang Thi Bui⁴  Srinivasan Ramasamy⁵  Hsiao-feng Lo⁶  Bharath Krishnan⁷  Mao-sen Liu⁸  Long-fang O. Chen⁸  Ramakrishnan Nair⁹  Miao-rong Yan^1,10  Jo-yi Yen^1,10 

[1] Biotechnology/Molecular Breeding, World Vegetable Center, 60 Yi Min Liao, 74151, Shanhua, Tainan, Taiwan;Department of Bio-Industrial Mechatronics Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 106, Taipei, Taiwan;Depertment of Agronomy, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 106, Taipei, Taiwan;Division of Agricultural Biodiversity, Plant Resources Center, Plant Resources Center, Vietnam Academy of Agriculture Sciences, An Khanh, Hoai Duc, Ha Noi, Vietnam;Entomology, World Vegetable Center, 60 Yi Min Liao, 74151, Shanhua, Tainan, Taiwan;Horticulture and Landscape Architecture / Horticulture Section, Experimental Farm, College of Bio-Resources and Agriculture National Taiwan University, No. 1, Sec. 4, Roosevelt Road, 106, Taipei, Taiwan;Information Technology, World Vegetable Center, 60 Yi Min Liao, 74151, Shanhua, Tainan, Taiwan;Institute of Plant and Microbial Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, 115, Taipei, Taiwan;Legume Breeding, World Vegetable Center South Asia, ICRISAT Campus, Patancheru, 502 324, Hyderabad, Telangana, India;Legume Breeding, World Vegetable Center, 60 Yi Min Liao, 74151, Shanhua, Tainan, Taiwan;

关键词: Quantitative Trait Locus;    Recombinant Inbred Line;    Quantitative Trait Locus Analysis;    Single Nucleotide Polymorphism Marker;    Quantitative Trait Locus Locus;   

DOI  :  10.1186/s12870-016-0847-8

 received in 2016-04-05, accepted in 2016-07-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBruchid beetles are an important storage pest of grain legumes. Callosobruchus sp. infect mungbean (Vigna radiata) at low levels in the field, multiply during grain storage and can destroy seed stocks in a few months. Resistance against bruchid beetles has been found in wild mungbean V. radiata var. sublobata TC1966 and in cultivated mungbean line V2802.ResultsBruchid resistance data were obtained from recombinant inbred line populations TC1966 (V. radiata var. sublobata) × NM92 (F12) and V2802 (V. radiata) × NM94 (F7). More than 6,000 single nucleotide polymorphic markers were generated through genotyping by sequencing (GBS) for each of these populations and were used to map bruchid resistance genes. One highly significant quantitative trait locus (QTL) associated with bruchid resistance was mapped to chromosome 5 on genetic maps of both populations, suggesting that TC1966 and V2802 contain the same resistance locus. Co-segregation of all markers associated with resistance indicated the presence of only one major resistance QTL on chromosome 5, while QTL analysis based on physical map positions of the markers suggested the presence of multiple QTLs on different chromosomes. The diagnostic capacity of the identified molecular markers located in the QTL to correctly predict resistance was up to 100 %.ConclusionsMolecular markers tightly linked to bruchid resistance loci of two different mungbean resistance sources were developed and validated. These markers are highly useful for developing resistant lines.

【 授权许可】

CC BY   
© The Author(s). 2016