In vitro effects of 0 to 120 Grays of irradiation on bone viability and release of growth factors

BMC Oral Health

Research Article

Masako Fujioka-Kobayashi¹  Eizaburo Kobayashi¹  Benoit Schaller¹  Kosaku Sawada²  Richard J. Miron³  Jens O. Brömme⁴ 

[1] Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, Bern, Switzerland;Department of Cranio Maxillofacial Surgery, Inselspital, University of Bern, Bern, Switzerland;The Nippon Dental University, School of Life Dentistry at Niigata, Advanced Research Center, Niigata, Japan;Department of Oral Surgery and Stomatology, Department of Periodontology, University of Bern, Bern, Switzerland;Department of Periodontology, College of Dental Medicine, Nova Southeastern University, 3200 South University Drive, 33328, Fort Lauderdale, Florida, USA;Department of Radiation Oncology, Inselspital, University of Bern, Bern, Switzerland;

关键词: Irradiation;    Bone cell death;    Grays;    Growth factor release;    Bone chips;   

DOI  :  10.1186/s12903-016-0241-9

 received in 2016-01-13, accepted in 2016-06-11,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundHigh dose radiation therapy is commonly used in maxillofacial surgeries to treat a number of head and neck tumors. Despite its widespread use, little information is available regarding the effects of irradiation on bone cell viability and release of growth factors following dose-dependent irradiation.MethodsBone samples were collected from porcine mandibular cortical bone and irradiated at doses of 0, 7.5, 15, 30, 60 and 120 Grays. Thereafter, cell viability was quantified, and the release of growth factors including TGFβ1, BMP2, VEGF, IL1β and RANKL were investigated over time.ResultsIt was observed that at only 7.5Gy of irradiation, over 85 % of cells were non-vital and by 60 Gy, all cells underwent apoptosis. Furthermore, over a 7-fold decrease in VEGF and a 2-fold decrease in TGFβ1 were observed following irradiation at all tested doses. Little change was observed for BMP2 and IL1β whereas RANKL was significantly increased for all irradiated samples.ConclusionsThese results demonstrate the pronounced effects of irradiation on bone-cell vitality and subsequent release of growth factors. Interestingly, the largest observed change in gene expression was the 7-fold decrease in VEGF protein following irradiation. Future research aimed at improving our understanding of bone following irradiation is necessary to further improve future clinical treatments.

【 授权许可】

CC BY   
© The Author(s). 2016