The transcriptome landscape of early maize meiosis

BMC Plant Biology

Research Article

Jaroslaw Pillardy¹  Qi Sun¹  Stefanie Dukowic-Schulze²  Changbin Chen²  Wojciech P Pawlowski³  Minghui Wang⁴  Thiruvarangan Ramaraj⁵  Ernest F Retzel⁵  Joann Mudge⁵  Anitha Sundararajan⁵  Andrew D Farmer⁵  Shahryar Kianian⁶ 

[1] Computational Biology Service Unit, Cornell University, 14850, Ithaca, NY, USA;Department of Horticultural Science, University of Minnesota, 55108, St. Paul, MN, USA;Department of Plant Breeding and Genetics, Cornell University, 14850, Ithaca, NY, USA;Department of Plant Breeding and Genetics, Cornell University, 14850, Ithaca, NY, USA;Computational Biology Service Unit, Cornell University, 14850, Ithaca, NY, USA;National Center for Genome Resources, 87505, Santa Fe, NM, USA;USDA-ARS Cereal Disease Laboratory, University of Minnesota, 55108, St. Paul, MN, USA;

关键词: Maize;    Meiosis;    Meiocytes;    Mitochondria;    RNA-seq;    Transcriptome;   

DOI  :  10.1186/1471-2229-14-118

 received in 2013-10-30, accepted in 2014-04-28,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundA major step in the higher plant life cycle is the decision to leave the mitotic cell cycle and begin the progression through the meiotic cell cycle that leads to the formation of gametes. The molecular mechanisms that regulate this transition and early meiosis remain largely unknown. To gain insight into gene expression features during the initiation of meiotic recombination, we profiled early prophase I meiocytes from maize (Zea mays) using capillary collection to isolate meiocytes, followed by RNA-seq.ResultsWe detected ~2,000 genes as preferentially expressed during early meiotic prophase, most of them uncharacterized. Functional analysis uncovered the importance of several cellular processes in early meiosis. Processes significantly enriched in isolated meiocytes included proteolysis, protein targeting, chromatin modification and the regulation of redox homeostasis. The most significantly up-regulated processes in meiocytes were processes involved in carbohydrate metabolism. Consistent with this, many mitochondrial genes were up-regulated in meiocytes, including nuclear- and mitochondrial-encoded genes. The data were validated with real-time PCR and in situ hybridization and also used to generate a candidate maize homologue list of known meiotic genes from Arabidopsis.ConclusionsTaken together, we present a high-resolution analysis of the transcriptome landscape in early meiosis of an important crop plant, providing support for choosing genes for detailed characterization of recombination initiation and regulation of early meiosis. Our data also reveal an important connection between meiotic processes and altered/increased energy production.

【 授权许可】

Unknown   
© Dukowic-Schulze et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.