Epigenetic regulation of the ELOVL6 gene is associated with a major QTL effect on fatty acid composition in pigs

Genetics Selection Evolution

Research

Estefânia Alves¹  Jordi Estellé²  Jorge AP Marchesi³  Maria Ballester⁴  Josep M Folch⁴  Manuel Revilla⁴  Jordi Corominas⁴  Anna Puig-Oliveras⁴ 

[1] Departamento de Mejora Genética Animal, INIA, Ctra. de la Coruña km. 7, 28040, Madrid, Spain;INRA, UMR 1313, Génétique Animale et Biologie Intégrative, 78352, Jouy-en-Josas F, France;AgroParisTech, UMR 1313 Génétique Animale et Biologie Intégrative, 78352, Jouy-en-Josas F, France;CEA, DSV/iRCM/SREIT/LREG, 78352, Jouy-en-Josas F, France;Plant and Animal Genomics, Centre de Recerca en Agrigenòmica (Consorci CSIC-IRTA-UAB-UB), Edifici CRAG, Campus UAB, 08193, Bellaterra, Barcelona, Spain;Plant and Animal Genomics, Centre de Recerca en Agrigenòmica (Consorci CSIC-IRTA-UAB-UB), Edifici CRAG, Campus UAB, 08193, Bellaterra, Barcelona, Spain;Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Campus UAB, 08193, Bellaterra, Barcelona, Spain;

关键词: Quantitative Trait Locus;    Bacterial Artificial Chromosome;    Methylation Level;    Bacterial Artificial Chromosome Clone;    Bacterial Artificial Chromosome Library;   

DOI  :  10.1186/s12711-015-0111-y

 received in 2014-09-18, accepted in 2015-03-04,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn previous studies on an Iberian x Landrace cross, we have provided evidence that supported the porcine ELOVL6 gene as the major causative gene of the QTL on pig chromosome 8 for palmitic and palmitoleic acid contents in muscle and backfat. The single nucleotide polymorphism (SNP) ELOVL6:c.-533C > T located in the promoter region of ELOVL6 was found to be highly associated with ELOVL6 expression and, accordingly, with the percentages of palmitic and palmitoleic acids in longissimus dorsi and adipose tissue. The main goal of the current work was to further study the role of ELOVL6 on these traits by analyzing the regulation of the expression of ELOVL6 and the implication of ELOVL6 polymorphisms on meat quality traits in pigs.ResultsHigh-throughput sequencing of BAC clones that contain the porcine ELOVL6 gene coupled to RNAseq data re-analysis showed that two isoforms of this gene are expressed in liver and adipose tissue and that they differ in number of exons and 3’UTR length. Although several SNPs in the 3’UTR of ELOVL6 were associated with palmitic and palmitoleic acid contents, this association was lower than that previously observed with SNP ELOVL6:c.-533C > T. This SNP is in full linkage disequilibrium with SNP ELOVL6:c.-394G > A that was identified in the binding site for estrogen receptor alpha (ERα). Interestingly, the ELOVL6:c.-394G allele is associated with an increase in methylation levels of the ELOVL6 promoter and with a decrease of ELOVL6 expression. Therefore, ERα is clearly a good candidate to explain the regulation of ELOVL6 expression through dynamic epigenetic changes in the binding site of known regulators of ELOVL6 gene, such as SREBF1 and SP1.ConclusionsOur results strongly suggest the ELOVL6:c.-394G > A polymorphism as the causal mutation for the QTL on pig chromosome 8 that affects fatty acid composition in pigs.

【 授权许可】

Unknown   
© Corominas et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.