Selection of oleaginous yeasts for fatty acid production

BMC Biotechnology

Research Article

Dirk Martens¹  René H. Wijffels²  Christien Lokman³  Eric van de Zilver³  Linda Peters³  Nick van Biezen³  Nicole Jacobs-van Dreumel³  Dennis Lamers⁴ 

[1] Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129,, 6700 EV, Wageningen, The Netherlands;Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129,, 6700 EV, Wageningen, The Netherlands;University of Nordland, Faculty of Biosciences and Aquaculture, N-8049, Bodø, Norway;HAN BioCentre, University of Applied Sciences, P.O. Box 6960,, 6503 GL, Nijmegen, The Netherlands;HAN BioCentre, University of Applied Sciences, P.O. Box 6960,, 6503 GL, Nijmegen, The Netherlands;Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129,, 6700 EV, Wageningen, The Netherlands;

关键词: Oleaginous yeast;    Schwanniomyces occidentalis;    Lipid production;    TAG;   

DOI  :  10.1186/s12896-016-0276-7

 received in 2015-08-12, accepted in 2016-05-23,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundOleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-called second generation biofuels, which are based on non-food competing “waste carbon sources”.ResultsIn this study the selection of potentially new interesting oleaginous yeast strains is described. Important selection criteria were: a broad maximum temperature and pH range for growth (robustness of the strain), a broad spectrum of carbon sources that can be metabolized (preferably including C-5 sugars), a high total fatty acid content in combination with a low glycogen content and genetic accessibility.ConclusionsBased on these selection criteria, among 24 screened species, Schwanniomyces occidentalis (Debaromyces occidentalis) CBS2864 was selected as a promising strain for the production of high amounts of lipids.

【 授权许可】

CC BY   
© The Author(s). 2016