microRNA levels in paraffin-embedded indolent B-cell non-Hodgkin lymphoma tissues from patients chronically infected with hepatitis B or C virus

BMC Infectious Diseases

Research Article

Paola Tataseo¹  Federico De Angelis²  Sara Panfilio²  Alessandro Pulsoni²  Anna Rita Ciccaglione³  Cinzia Marcantonio³  Enea Spada³  Roberto Bruni³  Umbertina Villano³  Paolo Bianco⁴  Mara Riminucci⁴  Maria Elena Tosti⁵  Alfonso Mele⁶  Fabrizio Marcucci⁷ 

[1] ASL Avezzano-Sulmona, Transfusional Medicine and Molecular Biology Laboratory, Sulmona, Italy;Department of Cellular Biotechnologies and Hematology, Sapienza University, Rome, Italy;Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy;Department of Molecular Medicine, Sapienza University, Rome, Italy;National Centre for Epidemiology, Surveillance and Health Promotion, Istituto Superiore di Sanità, Rome, Italy;National Centre for Epidemiology, Surveillance and Health Promotion, Istituto Superiore di Sanità, Rome, Italy;Associazione Calabrese di Epatologia (ACE), Pellaro (RC), Italy;Scientific Directorate, Regina Elena Cancer Institute, Rome, Italy;

关键词: Lymphoma;    Hepatitis B Virus;    Hepatitis C Virus;    microRNA;    FFPE;   

DOI  :  10.1186/1471-2334-14-S5-S6

来源: Springer

【 摘 要 】

BackgroundEpidemiological evidence links Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) to B-cell non-Hodgkin lymphoma (B-NHL). These B-NHLs, particularly those associated with HCV, may represent a distinct sub-group with peculiar molecular features, including peculiar expression of microRNAs (miRs).The aim of the present study was to search for miRs whose level in indolent B-NHL tissues could be associated with HBV or HCV infection.MethodsFourteen formalin fixed paraffin embedded (FFPE) tissues from HBV+, HCV+ and HBV-/HCV- indolent B-NHL patients were analyzed for levels of 34 selected miRs by quantitative Real-Time PCR. Reactive lymph nodes (RLNs) from HBV-/HCV- patients were included as non-tumor control. Statistical analysis of output data included Pearson and Spearman correlation and Mann-Whitney test and were carried out by the STATA software.ResultsMiR-92a was decreased exclusively in HBV-/HCV- B-NHLs, while miR-30b was increased in HBV+ and HCV+ samples, though only the HCV+ achieved full statistical significance. Analysis of a small subset of B-NHLs belonging to the same histological subtype (Nodal Marginal Zone Lymphoma) highlighted three miRs associated with HCV infection (miR-223, miR-29a and miR-29b) and confirmed decreased level of miR-92a in HBV-/HCV- samples also when considering this restricted B-NHL group.ConclusionsAlthough caution is needed due to the limited number of analyzed samples, overall the results suggest that differences at the miR expression level exist between indolent B-NHLs developed in patients with or without HBV or HCV infection. The identification of three further miRs associated with HCV by analyzing histologically homogeneous samples suggests that variations of miR levels possibly associated with HBV or HCV may be obscured by the tissue-specific variability of miR level associated with the different histological subtypes of B-NHL. Thus, the identification of further miRs will require, in addition to an increased sample size, the comparison of B-NHL tissues with the same histological classification.

【 授权许可】

CC BY   
© Bruni et al.; licensee BioMed Central Ltd. 2014