Identification of promising host-induced silencing targets among genes preferentially transcribed in haustoria of Puccinia

BMC Genomics

Research Article

Naeh L. Klages-Mundt¹  Samantha I. Downey²  Michael Pumphrey²  Chuntao Yin³  Scot H. Hulbert³  Sowmya Ramachandran³  Les J. Szabo⁴  Xianming Chen⁵ 

[1] Department of Biology, Carleton College, One North College St., 55057, Northfield, MN, USA;Department of Crop and Soil Sciences, Washington State University, 99164-6430, Pullman, WA, USA;Department of Plant Pathology, Washington State University, 99164-6430, Pullman, WA, USA;US Department of Agriculture, Agricultural Research Service, Cereal Disease Laboratory, 55108, St. Paul, MN, USA;US Department of Agriculture, Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research Unit, 99164-6430, Pullman, WA, USA;

关键词: Puccinia;    Haustorium;    Host-induced gene silencing;    Barley stripe mosaic virus;    Virus-induced gene silencing;   

DOI  :  10.1186/s12864-015-1791-y

 received in 2015-02-19, accepted in 2015-07-22,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe cereal rust fungi are destructive pathogens that affect grain production worldwide. Although the genomic and transcript sequences for three Puccinia species that attack wheat have been released, the functions of large repertories of genes from Puccinia still need to be addressed to understand the infection process of these obligate parasites. Host-induced gene silencing (HIGS) has emerged a useful tool to examine the importance of rust fungus genes while growing within host plants. In this study, HIGS was used to test genes from Puccinia with transcripts enriched in haustoria for their ability to interfere with full development of the rust fungi.ResultsApproximately 1200 haustoria enriched genes from Puccinia graminis f. sp. tritici (Pgt) were identified by comparative RNA sequencing. Virus-induced gene silencing (VIGS) constructs with fragments of 86 Puccinia genes, were tested for their ability to interfere with full development of these rust fungi. Most of the genes tested had no noticeable effects, but 10 reduced Pgt development after co-inoculation with the gene VIGS constructs and Pgt. These included a predicted glycolytic enzyme, two other proteins that are probably secreted and involved in carbohydrate or sugar metabolism, a protein involved in thiazol biosynthesis, a protein involved in auxin biosynthesis, an amino acid permease, two hypothetical proteins with no conserved domains, a predicted small secreted protein and another protein predicted to be secreted with similarity to bacterial proteins involved in membrane transport. Transient silencing of four of these genes reduced development of P. striiformis (Pst), and three of also caused reduction of P. triticina (Pt) development.ConclusionsPartial suppression of transcripts involved in a large variety of biological processes in haustoria cells of Puccinia rusts can disrupt their development. Silencing of three genes resulted in suppression of all three rust diseases indicating that it may be possible to engineer durable resistance to multiple rust pathogens with a single gene in transgenic wheat plants for sustainable control of cereal rusts.

【 授权许可】

Unknown   
© Yin et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.