Metaproteomic analysis of ratoon sugarcane rhizospheric soil

BMC Microbiology

Research Article

Rui Lin¹  Jun Chen²  Ruiyu Lin²  Aijia Zhang²  Mingming Zhou²  Haibin Wang²  Wenxiong Lin²  Sheng Lin²  Linkun Wu²  Zhixing Zhang² 

[1] College of Oceanography and Environmental Science, Xiamen University, 361005, Xiamen, Fujian, P. R. China;Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, P. R. China;Agroecological Institute, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, P. R. China;

关键词: CLPP;    2D-electrophoresis;    Soil enzyme;    Soil metaproteomics;    Soil protein extraction;    Sugarcane;   

DOI  :  10.1186/1471-2180-13-135

 received in 2013-02-02, accepted in 2013-05-30,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe current study was undertaken to elucidate the mechanism of yield decline in ratoon sugarcane using soil metaproteomics combined with community level physiological profiles (CLPP) analysis.ResultsThe available stalk number, stalk diameter, single stalk weight and theoretical yield of ratoon cane (RS) were found to be significantly lower than those of plant cane (NS). The activities of several carbon, nitrogen and phosphorus processing enzymes, including invertase, peroxidase, urease and phosphomonoesterase were found to be significantly lower in RS soil than in NS soil. BIOLOG analysis indicated a significant decline in average well-color development (AWCD), Shannon’s diversity and evenness indices in RS soil as compared to NS soil. To profile the rhizospheric metaproteome, 109 soil protein spots with high resolution and repeatability were successfully identified. These proteins were found to be involved in carbohydrate/energy, amino acid, protein, nucleotide, auxin and secondary metabolisms, membrane transport, signal transduction and resistance, etc. Comparative metaproteomics analysis revealed that 38 proteins were differentially expressed in the RS soil as compared to the control soil or NS soil. Among these, most of the plant proteins related to carbohydrate and amino acid metabolism and stress response were up-regulated in RS soil. Furthermore, several microbial proteins related to membrane transport and signal transduction were up-regulated in RS soil. These proteins were speculated to function in root colonization by microbes.ConclusionsOur experiments revealed that sugarcane ratooning practice induced significant changes in the soil enzyme activities, the catabolic diversity of microbial community, and the expression level of soil proteins. They influenced the biochemical processes in the rhizosphere ecosystem and mediated the interactions between plants and soil microbes.

【 授权许可】

CC BY   
© Lin et al.; licensee BioMed Central Ltd. 2013