Genome-wide comparative analysis of NBS-encoding genes between Brassica species and Arabidopsis thaliana

BMC Genomics

Research Article

Rui Qin¹  Jingyin Yu²  Wei Hua²  Fengqi Zhang²  Junyan Huang²  Chaobo Tong²  Xiaohui Cheng²  Caihua Dong²  Shengyi Liu²  Sadia Tehrim²  Yanqiu Zhou³ 

[1] Engineering Research Center of Protection and Utilization for Biological Resources in Minority Regions, South-Central University for Nationalities, 473061, Wuhan, China;Key Laboratory of Biology and Genetic Improvement of Oil crops, the Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, 430062, Wuhan, China;Key Laboratory of Biology and Genetic Improvement of Oil crops, the Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, 430062, Wuhan, China;Engineering Research Center of Protection and Utilization for Biological Resources in Minority Regions, South-Central University for Nationalities, 473061, Wuhan, China;

关键词: Brassica species;    Disease resistance gene;    Nucleotide binding site;    Tandem duplication;    Whole genome duplication;   

DOI  :  10.1186/1471-2164-15-3

 received in 2013-06-30, accepted in 2013-12-30,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPlant disease resistance (R) genes with the nucleotide binding site (NBS) play an important role in offering resistance to pathogens. The availability of complete genome sequences of Brassica oleracea and Brassica rapa provides an important opportunity for researchers to identify and characterize NBS-encoding R genes in Brassica species and to compare with analogues in Arabidopsis thaliana based on a comparative genomics approach. However, little is known about the evolutionary fate of NBS-encoding genes in the Brassica lineage after split from A. thaliana.ResultsHere we present genome-wide analysis of NBS-encoding genes in B. oleracea, B. rapa and A. thaliana. Through the employment of HMM search and manual curation, we identified 157, 206 and 167 NBS-encoding genes in B. oleracea, B. rapa and A. thaliana genomes, respectively. Phylogenetic analysis among 3 species classified NBS-encoding genes into 6 subgroups. Tandem duplication and whole genome triplication (WGT) analyses revealed that after WGT of the Brassica ancestor, NBS-encoding homologous gene pairs on triplicated regions in Brassica ancestor were deleted or lost quickly, but NBS-encoding genes in Brassica species experienced species-specific gene amplification by tandem duplication after divergence of B. rapa and B. oleracea. Expression profiling of NBS-encoding orthologous gene pairs indicated the differential expression pattern of retained orthologous gene copies in B. oleracea and B. rapa. Furthermore, evolutionary analysis of CNL type NBS-encoding orthologous gene pairs among 3 species suggested that orthologous genes in B. rapa species have undergone stronger negative selection than those in B .oleracea species. But for TNL type, there are no significant differences in the orthologous gene pairs between the two species.ConclusionThis study is first identification and characterization of NBS-encoding genes in B. rapa and B. oleracea based on whole genome sequences. Through tandem duplication and whole genome triplication analysis in B. oleracea, B. rapa and A. thaliana genomes, our study provides insight into the evolutionary history of NBS-encoding genes after divergence of A. thaliana and the Brassica lineage. These results together with expression pattern analysis of NBS-encoding orthologous genes provide useful resource for functional characterization of these genes and genetic improvement of relevant crops.

【 授权许可】

Unknown   
© Yu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.