Reelin expression during embryonic development of the pig brain

BMC Neuroscience

Research Article

Ida E Holm¹  Annette Søndergaard²  Anders L Nielsen²  Marianne G Johansen²  Karsten B Nielsen²  Arne L Jørgensen²  Kirsten Schauser³  Morten Vejlsted³ 

[1] Department of Pathology, Aalborg Hospital, Aarhus University Hospital, Aalborg, Denmark;Institute of Human Genetics, University of Aarhus, Bartholin Building 1240, Wilhelm Meyers Allé, 8000, Aarhus C, Denmark;University of Copenhagen, IBHV, Groennegaardsvej 7, 1870, Frederiksberg C, Denmark;

关键词: Marginal Zone;    Neuronal Migration;    Cortical Plate;    Reelin Expression;    Reelin Gene;   

DOI  :  10.1186/1471-2202-11-75

 received in 2009-10-18, accepted in 2010-06-15,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundReelin is an extracellular glycoprotein of crucial importance in the developmental organisation of neurons in the mammalian cerebral cortex and other laminated brain regions. The pig possesses a gyrencephalic brain that bears resemblance to the human brain. In order to establish an animal model for neuronal migration disorders in the pig, we have studied the expression pattern and structure of Reelin during pig brain development.ResultsWe determined the sequence of pig Reelin mRNA and protein and identified a high degree of homology to human Reelin. A peak in Reelin mRNA and protein expression is present during the period of major neurogenesis and neuronal migration. This resembles observations for human brain development. Immunohistochemical analysis showed the highest expression of Reelin in the Cajal-Reztius cells of the marginal zone, in resemblance with observations for the developing brain in humans and other mammalian species.ConclusionsWe conclude that the pig might serve as an alternative animal model to study Reelin functions and that manipulation of the pig Reelin could allow the establishment of an animal model for human neuronal migration disorders.

【 授权许可】

CC BY   
© Nielsen et al; licensee BioMed Central Ltd. 2010