Characterization of the age-dependent intervertebral disc changes in rabbit by correlation between MRI, histology and gene expression

BMC Musculoskeletal Disorders

Research Article

Julie Lesoeur¹  Jérôme Guicheux¹  Martial Masson¹  Marion Fusellier²  Gaël Grimandi³  Johann Clouet³  Marianne Pot-Vaucel⁴  Borhane H Fellah⁵  Olivier Gauthier⁵  Claire Vinatier⁶  Yves Maugars⁷  Yan Cherel⁸ 

[1] INSERM (Institut National de la Santé et de la Recherche Médicale) U791, LIOAD, Group Skeletal Tissue Engineering and Physiopathology (STEP), University of Nantes, F-44042, Nantes, France;PresUNAM, University of Nantes, Nantes, France;INSERM (Institut National de la Santé et de la Recherche Médicale) U791, LIOAD, Group Skeletal Tissue Engineering and Physiopathology (STEP), University of Nantes, F-44042, Nantes, France;PresUNAM, University of Nantes, Nantes, France;Department of Imagery, CRIP-VC, National Veterinary School (ONIRIS), F-44307, Nantes, France;INSERM (Institut National de la Santé et de la Recherche Médicale) U791, LIOAD, Group Skeletal Tissue Engineering and Physiopathology (STEP), University of Nantes, F-44042, Nantes, France;PresUNAM, University of Nantes, Nantes, France;Department of Pharmacy, University Hospital of Nantes, F-44093, Nantes, France;INSERM (Institut National de la Santé et de la Recherche Médicale) U791, LIOAD, Group Skeletal Tissue Engineering and Physiopathology (STEP), University of Nantes, F-44042, Nantes, France;PresUNAM, University of Nantes, Nantes, France;Department of Rheumatology, University Hospital of Nantes, F-44093, Nantes, France;INSERM (Institut National de la Santé et de la Recherche Médicale) U791, LIOAD, Group Skeletal Tissue Engineering and Physiopathology (STEP), University of Nantes, F-44042, Nantes, France;PresUNAM, University of Nantes, Nantes, France;Department of experimental Surgery, CRIP-VC, National Veterinary School (ONIRIS), F-44307, Nantes, France;INSERM (Institut National de la Santé et de la Recherche Médicale) U791, LIOAD, Group Skeletal Tissue Engineering and Physiopathology (STEP), University of Nantes, F-44042, Nantes, France;PresUNAM, University of Nantes, Nantes, France;Graftys SAS, Aix en Provence, France;PresUNAM, University of Nantes, Nantes, France;Department of Rheumatology, University Hospital of Nantes, F-44093, Nantes, France;PresUNAM, University of Nantes, Nantes, France;INRA UMR703, National Veterinary School (ONIRIS), F-44307, Nantes, France;

关键词: Nucleus Pulposus;    Annulus Fibrosus;    Nucleus Pulposus Cell;    Notochordal Cell;    Granular Change;   

DOI  :  10.1186/1471-2474-12-147

 received in 2011-03-29, accepted in 2011-07-04,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe present study was conducted to address whether the intervertebral disc of rabbit could be considered (i) as a valuable model to provide new insights into the tissue and cellular changes of Nucleus pulposus aging and (ii) as an appropriate tool to investigate the efficacy of Nucleus pulposus cell-based biotherapies.MethodsLumbar intervertebral disc from rabbits with increasing ages (1, 6 and 30 month-old) were compared by MRI and histological observation using Pfirrmann's grading and Boos' scoring respectively. The expression of transcripts (COL2A1, AGC1, COL1A1, MMP13, BMP2, MGP and p21) in Nucleus pulposus cells were analysed by quantitative real-time PCR.ResultsMRI analysis indicated an early age-dependent increase in the Pfirrmann's grading. Histological Boos' scoring was also increased. The analysis of transcript expression levels showed that COL2A1 and AGC1 were down-regulated as a function of age. Conversely, COL1A1, MMP-13, BMP-2, MGP and p21 were significantly up-regulated in the Nucleus pulposus cells of aged rabbit intervertebral disc.ConclusionsOur study describes the consistency of the rabbit as a model of intervertebral disc changes as a function of age by correlating tissue alteration with cellular modification measured.

【 授权许可】

Unknown   
© Clouet et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.