Brewers’ rice induces apoptosis in azoxymethane-induced colon carcinogenesis in rats via suppression of cell proliferation and the Wnt signaling pathway

BMC Complementary and Alternative Medicine

Research Article

Roselina Karim¹  Bee Ling Tan²  Norhaizan Mohd Esa³  Hazilawati Hamzah⁴  Heshu Sulaiman Rahman⁴ 

[1] Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;

关键词: Brewers’ rice;    Colon cancer;    Beta-catenin;    Ki-67;    Apoptosis;   

DOI  :  10.1186/1472-6882-14-304

 received in 2014-05-21, accepted in 2014-08-13,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundBrewers’ rice is locally known as temukut, is a byproduct of the rice milling process, and consists of broken rice, rice bran, and rice germ. Unlike rice bran, the health benefit of brewers’ rice has yet to be fully studied. Our present study aimed to identify the chemopreventive potential of brewers’ rice with colonic tumor formation and to examine further the mechanistic action of brewers’ rice during colon carcinogenesis.MethodsMale Sprague–Dawley rats were randomly divided into five groups: (G1) normal; (G2) azoxymethane (AOM) alone; and (G3), (G4), and (G5), which were AOM fed with 10%, 20%, and 40% (w/w) of brewers’ rice, respectively. Rats in group 2 to 5 were injected intraperitoneally with AOM (15 mg/kg body weight) once weekly for two weeks. Colon tumor incidence and multiplicity was assessed by hematoxylin and eosin (H&E) staining. The expression of β-catenin, cyclooxygenase-2 (COX-2), and Ki-67 was evaluated by immunohistochemical staining. The apoptosis-inducing activity was analyzed using a TUNEL assay. The data were analyzed using a one-way analysis of variance (ANOVA) with P-value<0.05 was considered significant.ResultsOverall analyses revealed that brewers’ rice reduced colon tumor incidence and multiplicity. The results from immunohistochemistry analysis also showed that brewers’ rice decreased the expression of β-catenin, COX-2, and Ki-67 in a dose-dependent manner. Furthermore, TUNEL analysis demonstrated that administration of brewers’ rice in AOM-induced rat colorectal cancer resulted in a dose-dependent increase in cell apoptosis.ConclusionsTaken together, our data suggested that brewers’ rice can inhibit cell proliferation, induce apoptosis, and suppress COX-2 and β-catenin expression via the Wnt signaling pathway and holds great promise in the field of chemoprevention as a dietary agent.

【 授权许可】

Unknown   
© Tan et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.