Transcriptomics analysis of the flowering regulatory genes involved in the herbicide resistance of Asia minor bluegrass (Polypogon fugax)

BMC Genomics

Research Article

Fengyan Zhou¹  Yong Zhang¹  Mei Wang¹  Tongchun Gao¹  Wei Tang² 

[1] Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, 230001, Hefei, China;State Key Laboratory of Rice Biology, China National Rice Research Institute, 311400, Hangzhou, China;

关键词: RNA sequencing;    Transcriptomics;    Herbicide resistance;    Polypogon fugax;    Clodinafop-propargyl;    Flowering;   

DOI  :  10.1186/s12864-017-4324-z

 received in 2017-07-19, accepted in 2017-11-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAsia minor bluegrass (Polypogon fugax, P. fugax), a weed that is both distributed across China and associated with winter crops, has evolved resistance to acetyl-CoA carboxylase (ACCase) herbicides, but the resistance mechanism remains unclear. The goal of this study was to analyze the transcriptome between resistant and sensitive populations of P. fugax at the flowering stage.ResultsPopulations resistant and susceptible to clodinafop-propargyl showed distinct transcriptome profiles. A total of 206,041 unigenes were identified; 165,901 unique sequences were annotated using BLASTX alignment databases. Among them, 5904 unigenes were classified into 58 transcription factor families. Nine families were related to the regulation of plant growth and development and to stress responses. Twelve unigenes were differentially expressed between the clodinafop-propargyl-sensitive and clodinafop-propargyl-resistant populations at the early flowering stage; among those unigenes, three belonged to the ABI3VP1, BHLH, and GRAS families, while the remaining nine belonged to the MADS family. Compared with the clodinafop-propargyl-sensitive plants, the resistant plants exhibited different expression pattern of these 12 unigenes.ConclusionThis study identified differentially expressed unigenes related to ACCase-resistant P. fugax and thus provides a genomic resource for understanding the molecular basis of early flowering.

【 授权许可】

CC BY   
© The Author(s). 2017