Molecular characteristics of Hepatitis B and chronic liver disease in a cohort of HB carriers from Bamako, Mali

BMC Infectious Diseases

Research Article

Marlin D Friesen¹  John D Groopman¹  Fatou Traoré²  Souleymane Diallo²  Stéphanie Villar³  Pierre Hainaut⁴  Guy Vernet⁵  Moussa Y Maiga⁶  Emmanuelle Gormally⁷ 

[1] Bloomberg School of Public Health, Johns Hopkins University, Baltimore, USA;Centre d’Infectiologie Charles Mérieux, Bamako, République du Mali;International Agency for Research on Cancer, Lyon, France;International Prevention Research Institute, Lyon, France;Laboratoire des Pathogènes Emergents Fondation Mérieux, Lyon, France;Centre Pasteur du Cameroun, Yaoundé, Cameroun;Service de Gastroentérologie et Hépatologie, Centre Hospitalier Universitaire Gabriel Touré, Bamako, République du Mali;Université de Lyon, UMRS 449 ; Laboratoire de Biologie générale, Université Catholique de Lyon ; Reproduction et développement comparé, EPHE, Lyon, France;

关键词: HBV;    Viral load;    Chronic carriage;    Fibrotest/Actitest;    TP53 R249S;    Mali;   

DOI  :  10.1186/s12879-015-0916-x

 received in 2014-09-24, accepted in 2015-03-27,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundHepatitis B (HB) infection is common in Mali. However, there is little information on molecular and biochemical characteristics of HB carriers.MethodsA group of 1466 adult volunteers was recruited in the district of Bamako. Confirmed HB carriers were tested for HB viral load by quantitative PCR and HBV was genotyped by sequencing of HBS. Fibrosis and hepatitis activity were measured using the Fibrotest-Actitest. A mutation of TP53 at codon 249 (R249S), specific for exposure to aflatoxin, was detected in cell-free DNA extracted from plasma.ResultsOverall, 276 subjects were HBsAg-positive (18.8%). Among 152 subjects tested for HBV load, 49 (32.2%) had over 104 copies/mL and 16 (10.5%) had levels below the limit of detection. The E genotype was found in 91.1% of carriers. Fibrotest scores ≥ F2 were observed in 52 subjects (35.4%). Actitest scores ≥ A2 were detected in 15 subjects (10.2%) and were correlated with Fibrotest scores (p = 0.0006). Among 105 subjects tested, 60% had detectable levels of R249S copies (>40 copies/mL plasma).ConclusionChronic HB carriage in adults in Bamako district is well over epidemic threshold. About 1/3 of carriers have moderate to severe liver fibrosis and 60% have detectable aflatoxin-related TP53 R249S mutation. These results support introduction of anti-HB therapies to reduce the progression towards severe liver disease.

【 授权许可】

Unknown   
© Traoré et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.