Arabidopsis leucine-rich repeat extensin (LRX) proteins modify cell wall composition and influence plant growth

BMC Plant Biology

Research Article

Antony Bacic¹  Isabel Moller²  Markus Pauly³  Benjamin M. Kuhn³  Grégory Mouille⁴  Emilie Gineau⁵  Christoph Ringli⁶  Marie-Therese Abdou⁶  Tohnyui Ndinyanka Fabrice⁶  Christian Draeger⁷  Beat Frey⁸ 

[1] ARC Centre of Excellence in Plant Cell Walls, School of Botany, the University of Melbourne, 3010, Parkville, Victoria, Australia;ARC Centre of Excellence in Plant Cell Walls, School of Botany, the University of Melbourne, 3010, Parkville, Victoria, Australia;Current address: The New Zealand Institute for Plant & Food Research Limited, 1142, Auckland, New Zealand;Department of Plant and Microbial Biology, UC Berkeley, 94720, Berkeley, CA, USA;INRA, Institut Jean-Pierre Bourgin, UMR1318 Saclay Plant Sciences, 78026, Versailles, France;INRA, Institut Jean-Pierre Bourgin, UMR1318 Saclay Plant Sciences, 78026, Versailles, France;AgroParisTech, Institut Jean-Pierre Bourgin, UMR1318 Saclay Plant Sciences, 78026, Versailles, France;Institute of Plant Biology, University of Zürich, Zollikerstr. 107, 8008, Zürich, Switzerland;Institute of Plant Biology, University of Zürich, Zollikerstr. 107, 8008, Zürich, Switzerland;Current address: Thermo Fisher Scientific, Neuhofstrasse 11, 4153, Reinach, Switzerland;Swiss Federal Research Institute WSL, 8903, Birmensdorf, Switzerland;

关键词: Arabidopsis;    Cell wall development;    Cell wall structures;    LRR-extensin;    LRX;   

DOI  :  10.1186/s12870-015-0548-8

 received in 2014-12-08, accepted in 2015-06-11,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundLeucine-rich repeat extensins (LRXs) are extracellular proteins consisting of an N-terminal leucine-rich repeat (LRR) domain and a C-terminal extensin domain containing the typical features of this class of structural hydroxyproline-rich glycoproteins (HRGPs). The LRR domain is likely to bind an interaction partner, whereas the extensin domain has an anchoring function to insolubilize the protein in the cell wall. Based on the analysis of the root hair-expressed LRX1 and LRX2 of Arabidopsis thaliana, LRX proteins are important for cell wall development. The importance of LRX proteins in non-root hair cells and on the structural changes induced by mutations in LRX genes remains elusive.ResultsThe LRX gene family of Arabidopsis consists of eleven members, of which LRX3, LRX4, and LRX5 are expressed in aerial organs, such as leaves and stem. The importance of these LRX genes for plant development and particularly cell wall formation was investigated. Synergistic effects of mutations with gradually more severe growth retardation phenotypes in double and triple mutants suggest a similar function of the three genes. Analysis of cell wall composition revealed a number of changes to cell wall polysaccharides in the mutants.ConclusionsLRX3, LRX4, and LRX5, and most likely LRX proteins in general, are important for cell wall development. Due to the complexity of changes in cell wall structures in the lrx mutants, the exact function of LRX proteins remains to be determined. The increasingly strong growth-defect phenotypes in double and triple mutants suggests that the LRX proteins have similar functions and that they are important for proper plant development.

【 授权许可】

Unknown   
© Draeger et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.