Effects of Litchi chinensis fruit isolates on prostaglandin E2 and nitric oxide production in J774 murine macrophage cells

BMC Complementary and Alternative Medicine

Research Article

Hongtao Lei¹  Hong Gao¹  Hong Wang¹  Yang Zhou¹  Yudong Shen¹  Yuanming Sun¹  Hui Huang¹  Ruili Yang¹ 

[1] Guangdong Provincial Key laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Wushan Road, 510642, Guangzhou, China;

关键词: Nitric Oxide;    Benzyl Alcohol;    PGE2 Production;    J774 Cell;    Bitter Gourd;   

DOI  :  10.1186/1472-6882-12-12

 received in 2011-03-03, accepted in 2012-03-01,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundLitchi chinensis is regarded as one of the 'heating' fruits in China, which causes serious inflammation symptoms to people.MethodsIn the current study, the effects of isolates of litchi on prostaglandin E2 (PGE2) and nitric oxide (NO) production in J774 murine macrophage cells were investigated.ResultsThe AcOEt extract (EAE) of litchi was found effective on stimulating PGE2 production, and three compounds, benzyl alcohol, hydrobenzoin and 5-hydroxymethyl-2-furfurolaldehyde (5-HMF), were isolated and identified from the EAE. Benzyl alcohol caused markedly increase in PGE2 and NO production, compared with lipopolysaccharide (LPS) as positive control, and in a dose-dependent manner. Hydrobenzoin and 5-HMF were found in litchi for the first time, and both of them stimulated PGE2 and NO production moderately in a dose-dependent manner. Besides, regulation of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) mRNA expression and NF-κB (p50) activation might be involved in mechanism of the stimulative process.ConclusionThe study showed, some short molecular compounds in litchi play inflammatory effects on human.

【 授权许可】

Unknown   
© Zhou et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.