Retama monosperma n-hexane extract induces cell cycle arrest and extrinsic pathway-dependent apoptosis in Jurkat cells

BMC Complementary and Alternative Medicine

Research Article

Saaïd Amzazi¹  Youssef Bakri¹  Nawel Merghoub¹  Clara Aceves-Luquero²  Lamiae Belayachi³  Priam Villalonga⁴  Silvia Fernández de Mattos⁴ 

[1] Biochemistry Immunology Laboratory, Faculty of Sciences, Mohammed V-Agdal University, Rabat, Morocco;Cancer Cell Biology Group, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Edifici Cientificotècnic, Ctra Km 7,5, Valldemossa, Illes Balears, Spain;Cancer Cell Biology Group, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Edifici Cientificotècnic, Ctra Km 7,5, Valldemossa, Illes Balears, Spain;Biochemistry Immunology Laboratory, Faculty of Sciences, Mohammed V-Agdal University, Rabat, Morocco;Cancer Cell Biology Group, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Edifici Cientificotècnic, Ctra Km 7,5, Valldemossa, Illes Balears, Spain;Departament de Biologia Fonamental, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, Edifici Cientificotècnic, Ctra Km 7,5, Valldemossa, Illes Balears, Spain;

关键词: Retama monosperma;    Acute T-cell leukemia;    Cytotoxicity;    Apoptosis;    Bioactive compounds;   

DOI  :  10.1186/1472-6882-14-38

 received in 2013-10-02, accepted in 2014-01-14,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRetama monosperma L. (Boiss.) or Genista monosperma L. (Lam.), locally named as “R’tam”, is an annual and spontaneous plant belonging to the Fabaceae family. In Morocco, Retama genus is located in desert regions and across the Middle Atlas and it has been widely used in traditional medicine in many countries. In this study, we show that Retama monosperma hexane extract presents significant anti-leukemic effects against human Jurkat cells.MethodsHuman Jurkat cells, together with other cell lines were screened with different concentrations of Retama monosperma hexane extract at different time intervals. Growth inhibition was determined using luminescent-based viability assays. Cell cycle arrest and apoptosis were measured by flow cytometry analysis. Combined caspase 3 and 7 activities were measured using luminometric caspase assays and immunoblots were performed to analyze expression of relevant pro- and anti-apoptotic proteins. GC-MS were used to determine the chemical constituents of the active extract.ResultsRetama monosperma hexane extract (Rm-HE) showed significant cytotoxicity against Jurkat cells, whereas it proved to be essentially ineffective against both normal mouse fibroblasts (NIH3T3) and normal lymphocytes (TK-6). Cytometric analysis indicated that Rm-HE promoted cell cycle arrest and apoptosis induction accompanied by DNA damage induction indicated by an increase in p-H2A.X levels. Rm-HE induced apoptosis was partially JNK-dependent and characterized by an increase in Fas-L levels together with activation of caspases 8, 3, 7 and 9, whereas neither the pro-apoptotic nor anti-apoptotic mitochondrial membrane proteins analyzed were significantly altered. Chemical identification analysis indicated that α-linolenic acid, campesterol, stigmasterol and sitosterol were the major bioactive components within the extract.ConclusionsOur data suggest that bioactive compounds present in Rm-HE show significant anti leukemic activity inducing cell cycle arrest and cell death that operates, at least partially, through the extrinsic apoptosis pathway.

【 授权许可】

Unknown   
© Belayachi et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.