Jasmonates act positively in adventitious root formation in petunia cuttings

BMC Plant Biology

Research Article

Bettina Hause¹  Anne Muchow²  Daniela Guthörl³  Sandra Lischweski⁴ 

[1] Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D06120, Halle/Salle, Germany;Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D06120, Halle/Salle, Germany;Present address: IDT Biologika GmbH, Am Pharmapark, D06861, Dessau-Roßlau, Germany;Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D06120, Halle/Salle, Germany;Present address: Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, CH-8008, Zürich, Switzerland;Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D06120, Halle/Salle, Germany;Present address: Interdisziplinäres Stoffwechsel-Centrum, Charité, Augustenburger Platz 1, D13353, Berlin, Germany;

关键词: Adventitious root formation;    Auxin;    Cell wall invertase;    Cuttings;    Ethylene;    Jasmonic acid;    JA-isoleucine;    Petunia hybrida;   

DOI  :  10.1186/s12870-015-0615-1

 received in 2015-05-04, accepted in 2015-09-12,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPetunia is a model to study the process of adventitious root (AR) formation on leafy cuttings. Excision of cuttings leads to a transient increase in jasmonates, which is regarded as an early, transient and critical event for rooting. Here, the role of jasmonates in AR formation on petunia cuttings has been studied by a reverse genetic approach.ResultsTo reduce the endogenous levels of jasmonates, transgenic plants were generated expressing a Petunia hybrida ALLENE OXIDE CYCLASE (PhAOC)-RNAi construct. The transgenic plants exhibited strongly reduced PhAOC transcript and protein levels as well as diminished accumulation of cis-12-oxo-phytodienoic acid, jasmonic acid and jasmonoyl-isoleucine after wounding in comparison to wild type and empty vector expressing plants. Reduced levels of endogenous jasmonates resulted in formation of lower numbers of ARs. However, this effect was not accompanied by altered levels of auxin and aminocyclopropane carboxylate (ACC, precursor of ethylene) or by impaired auxin and ethylene-induced gene expression. Neither activity of cell-wall invertases nor accumulation of soluble sugars was altered by jasmonate deficiency.ConclusionsDiminished numbers of AR in JA-deficient cuttings suggest that jasmonates act as positive regulators of AR formation in petunia wild type. However, wound-induced rise in jasmonate levels in petunia wild type cuttings seems not to be causal for increased auxin and ethylene levels and for sink establishment.

【 授权许可】

CC BY   
© Lischweski et al. 2015