Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.)

BMC Genomics

Research Article

Steven B. Cannon¹  John H. Hill²  Steven A. Whitham²  Kathleen Martin³  Jugpreet Singh⁴ 

[1] Department of Agronomy, Iowa State University, 50011, Ames, IA, USA;USDA-ARS, Corn Insects and Crop Genetics Research Unit, Crop Genome Informatics Laboratory, Iowa State University, 50011, Ames, IA, USA;Department of Plant Pathology and Microbiology, Iowa State University, 50011, Ames, USA;Department of Plant Pathology, Kansas State University, 66506, Manhattan, KS, USA;ORISE Fellow, USDA-ARS, Corn Insects and Crop Genetics Research Unit, 50011, Ames, IA, USA;

关键词: Gene expression;    Gene regulation;    Host-virus interaction;    Regulatory changes;    Phaseolus vulgaris;    Bean common mosaic virus;   

DOI  :  10.1186/s12864-016-2976-8

 received in 2016-05-15, accepted in 2016-07-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. The molecular responses in Phaseolus to BCMV infection have not yet been well characterized.ResultsWe report the transcriptional responses of a widely susceptible variety of common bean (Phaseolus vulgaris L., cultivar ‘Stringless green refugee’) to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Iowa causes moderate symptoms and large transcriptional responses, and the newly identified strain (Strain 2 or S2) causes severe symptoms and moderate transcriptional responses. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns.ConclusionsWe identified differential host transcriptome response after infection of two different strains of Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.). Virus infection initiated a suite of changes in gene expression level and patterns in the host plants. Pathways related to defense, gene regulation, metabolic processes, photosynthesis were specifically altered after virus infection. Results presented in this study can increase the understanding of host-pathogen interactions and provide resources for further investigations of the biological mechanisms in BCMV infection and defense.

【 授权许可】

CC BY   
© The Author(s). 2016