Differentiation in MALDI-TOF MS and FTIR spectra between two closely related species Acidovorax oryzae and Acidovorax citrulli

BMC Microbiology

Research Article

Guoxing Wu¹  Guochang Sun²  Baoping Liu³  Guanlin Xie³  Qing Zhou³  Muhammad Ibrahim³  Hongye Li³  Bin Li³  Yanli Wang⁴ 

[1]College of Plant Protection, Yunnan Agricultural University, 650201, Kunming, China

[2]State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China

[3]State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310029, Hangzhou, China

[4]State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, 310029, Hangzhou, China

[5]State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, 310021, Hangzhou, China

关键词: Melon;    FTIR Spectroscopy;    Plant Pathogenic Bacterium;    False Identification;    Fame Profile;   

DOI  :  10.1186/1471-2180-12-182

 received in 2012-05-15, accepted in 2012-07-23,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundTwo important plant pathogenic bacteria Acidovorax oryzae and Acidovorax citrulli are closely related and often not easy to be differentiated from each other, which often resulted in a false identification between them based on traditional methods such as carbon source utilization profile, fatty acid methyl esters, and ELISA detection tests. MALDI-TOF MS and Fourier transform infrared (FTIR) spectra have recently been successfully applied in bacterial identification and classification, which provide an alternate method for differentiating the two species.ResultsCharacterization and comparison of the 10 A. oryzae strains and 10 A. citrulli strains were performed based on traditional bacteriological methods, MALDI-TOF MS, and FTIR spectroscopy. Our results showed that the identity of the two closely related plant pathogenic bacteria A. oryzae and A. citrulli was able to be confirmed by both pathogenicity tests and species-specific PCR, but the two species were difficult to be differentiated based on Biolog and FAME profile as well as 16 S rRNA sequence analysis. However, there were significant differences in MALDI-TOF MS and FTIR spectra between the two species of Acidovorax. MALDI-TOF MS revealed that 22 and 18 peaks were specific to A. oryzae and A. citrulli, respectively, while FTIR spectra of the two species of Acidovorax have the specific peaks at 1738, 1311, 1128, 1078, 989 cm-1 and at 1337, 968, 933, 916, 786 cm-1, respectively.ConclusionsThis study indicated that MALDI-TOF MS and FTIR spectra may give a new strategy for rapid bacterial identification and differentiation of the two closely related species of Acidovorax.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.