Genome comparisons provide insights into the role of secondary metabolites in the pathogenic phase of the Photorhabdus life cycle

BMC Genomics

Research Article

Rahul Sharma¹  Marco Thines²  Bagdevi Mishra²  Deepak K. Gupta²  Timothy P. Stinear³  Nicholas J. Tobias⁴  Helge B. Bode⁵ 

[1] Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, Senckenberganlage 25, 60325, Frankfurt am Main, Germany;Biodiversity and Climate Research Centre (BiK-F), Senckenberg Gesellschaft für Naturforschung, Senckenberganlage 25, 60325, Frankfurt am Main, Germany;Fachbereich Biowissenschaften, Institut für Ökologie, Evolution und Diversität, Goethe Universität Frankfurt, Max-von-Laue-Str. 13, 60438, Frankfurt am Main, Germany;Department of Microbiology and Immunology, University of Melbourne, at the Doherty Institute for Infection and Immunity, 3010, Parkville, VIC, Australia;Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe Universität Frankfurt, Frankfurt am Main, Germany;Fachbereich Biowissenschaften, Merck Stiftungsprofessur für Molekulare Biotechnologie, Goethe Universität Frankfurt, Frankfurt am Main, Germany;Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt, Frankfurt am Main, Germany;

关键词: Photorhabdus;    Sequencing;    Secondary metabolites;    Symbiosis;   

DOI  :  10.1186/s12864-016-2862-4

 received in 2016-02-06, accepted in 2016-06-29,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundBacteria within the genus Photorhabdus maintain mutualistic symbioses with nematodes in complicated lifecycles that also involves insect pathogenic phases. Intriguingly, these bacteria are rich in biosynthetic gene clusters that produce compounds with diverse biological activities. As a basis to better understand the life cycles of Photorhabdus we sequenced the genomes of two recently discovered representative species and performed detailed genomic comparisons with five publically available genomes.ResultsHere we report the genomic details of two new reference Photorhabdus species. By then conducting genomic comparisons across the genus, we show that there are several highly conserved biosynthetic gene clusters. These clusters produce a range of bioactive small molecules that support the pathogenic phase of the integral relationship that Photorhabdus maintain with nematodes.ConclusionsPhotorhabdus contain several genetic loci that allow them to become specialist insect pathogens by efficiently evading insect immune responses and killing the insect host.

【 授权许可】

CC BY   
© The Author(s). 2016