Anti-carcinogenic effects of the phenolic-rich extract from abnormal Savda Munziq in association with its cytotoxicity, apoptosis-inducing properties and telomerase activity in human cervical cancer cells (SiHa)

BMC Complementary and Alternative Medicine

Research Article

Guzalnur Abdixkur¹  Li Hua¹  Fatima Mijit¹  Guzalnur Abliz¹  Tangnur Ablimit¹  Nurmuhammat Amat²  Halmurat Upur² 

[1] Fifth Department of Gynecology, Affiliated Tumor Hospital, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China;Traditional Uighur Medicine Institute, Xinjiang Medical University, 393 Medical University Road, 830011, Urumqi, Xinjiang, PR China;

关键词: Phenolic rich extracts of ASMq;    Human cervical cancer;    SiHa cells;    Apoptosis;    Telomerase;   

DOI  :  10.1186/s12906-015-0530-x

 received in 2014-07-10, accepted in 2015-01-16,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAbnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment cancer. The polyphenol is main compounds contained in ASMq preparation responsible for anticancer effect of ASMq.MethodsIn this study,Real-time quantitative Polymerase Chain Reaction (RT-PCR) assay, MTT assay and flow cytometry were used to investigate the effect of polyphenol of ASMq on cell viability and the potential of the phenolic rich extracts of ASMq to induce apoptosis in human cervical cancer cells SiHa and its effects on telomerase activity were investigated. Cellular morphological change was observed by phase contrast microscopy. The MTT cell viability data revealed that treatment with phenolic rich extracts at 75 ~ 175 μg/ml significantly inhibited the viability and proliferation of cells, and these effects occurred in a concentration-dependent manner and time dependent manner (P < 0.01).ResultsThe phenolic rich extracts can induce apoptosis of SiHa cells, can increase the apoptosis rate in a concentration-dependent manner and time dependent manner (P < 0.01). Growth inhibition and apoptosis induction by phenolic rich extracts treatment on SiHa cells was associated with down-regulation of anti-apoptotic Bcl-2 expression and telomerase (P < 0.05) and Survivin expression. In addition, phenolic rich extracts exerted a dose-dependent induction of FHIT expression.ConclusionThese results suggest that phenolic rich extracts may have anti-tumor effects in human cervical cancer through cytotoxicity, apoptosis-inducing properties and telomerase activity.

【 授权许可】

Unknown   
© Abliz et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.