Deciphering the genomic structure, function and evolution of carotenogenesis related phytoene synthases in grasses

BMC Genomics

Research Article

Antonio Blanco¹  Ingrid Mercier²  Hélène Bergès²  Jérôme Salse³  Audrey Chosson³  Caroline Pont³  Charles Poncet³  Florent Murat³  Véronique Gautier³  Philippe Lecomte³  Bianca Dibari⁴ 

[1] DIBCA - Department of Agro-Forestry and Enviromental Biology and Chemistry, Sezione di Genetica e Miglioramento Genetico, Via Amendola 165/A, 70126, Bari, Italy;INRA - Centre National de Ressources Génomiques Végétales (CNRGV), Chemin de Borde Rouge BP 52627, 31326, Castanet Tolosan, cedex, France;INRA - UMR 1095 ‘Génétique Diversité Ecophysiologie des Céréales’ (GDEC), 5 Chemin de Beaulieu, 63100, Clermont-Ferrand, France;INRA - UMR 1095 ‘Génétique Diversité Ecophysiologie des Céréales’ (GDEC), 5 Chemin de Beaulieu, 63100, Clermont-Ferrand, France;DIBCA - Department of Agro-Forestry and Enviromental Biology and Chemistry, Sezione di Genetica e Miglioramento Genetico, Via Amendola 165/A, 70126, Bari, Italy;

关键词: Carotenoids;    Phytoene synthase;    Wheat;    Intron loss;    Abiotic stress;    Evolution;   

DOI  :  10.1186/1471-2164-13-221

 received in 2012-02-09, accepted in 2012-06-06,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCarotenoids are isoprenoid pigments, essential for photosynthesis and photoprotection in plants. The enzyme phytoene synthase (PSY) plays an essential role in mediating condensation of two geranylgeranyl diphosphate molecules, the first committed step in carotenogenesis. PSY are nuclear enzymes encoded by a small gene family consisting of three paralogous genes (PSY 1-3) that have been widely characterized in rice, maize and sorghum.ResultsIn wheat, for which yellow pigment content is extremely important for flour colour, only PSY 1 has been extensively studied because of its association with QTLs reported for yellow pigment whereas PSY 2 has been partially characterized. Here, we report the isolation of bread wheat PSY 3 genes from a Renan BAC library using Brachypodium as a model genome for the Triticeae to develop Conserved Orthologous Set markers prior to gene cloning and sequencing. Wheat PSY 3 homoeologous genes were sequenced and annotated, unravelling their novel structure associated with intron-loss events and consequent exonic fusions. A wheat PSY 3 promoter region was also investigated for the presence of cis-acting elements involved in the response to abscisic acid (ABA), since carotenoids also play an important role as precursors of signalling molecules devoted to plant development and biotic/abiotic stress responses. Expression of wheat PSYs in leaves and roots was investigated during ABA treatment to confirm the up-regulation of PSY 3 during abiotic stress.ConclusionsWe investigated the structural and functional determinisms of PSY genes in wheat. More generally, among eudicots and monocots, the PSY gene family was found to be associated with differences in gene copy numbers, allowing us to propose an evolutionary model for the entire PSY gene family in Grasses.

【 授权许可】

Unknown   
© Dibari et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.