Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis

BMC Infectious Diseases

Research Article

San-Yuan Chen¹  Pei-I Tsai²  Jui-Sheng Sun³  Lih-Tao Hsu⁴  Shu-Wei Huang⁵  Chih-Hsiang Fang⁵  Feng Huei Lin⁵  Jenny Zwei-Chieng Chang⁶  Hsin-Hsin Shen⁷  Yen-Chun Chen⁷ 

[1] Department of Materials Science and Engineering, National Chiao-Tung University, 30010, Hsinchu, Taiwan;Department of Materials Science and Engineering, National Chiao-Tung University, 30010, Hsinchu, Taiwan;Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 31040, Chutung, Hsinchu, Taiwan;Department of Orthopedic Surgery, College of Medicine, National Taiwan University, No. 1, Ren-Ai Rd, 10051, Taipei, Taiwan, Republic of China;Department of Orthopedic Surgery, National Taiwan University Hospital, No.7, Chung-Shan South Rd, 10002, Taipei, Taiwan, Republic of China;Biomimetic Systems Research Center, National Chiao-Tung University, 1001 University Road, 300, Hsinchu, Taiwan, Republic of China;Industrial Technology Research Institute, Rm. 635, Bldg. 53, No. 195, Sec. 4, Chung Hsing Rd, Chutung, Hsinchu, Taiwan;Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan;School of Dentistry, College of Medicine, National Taiwan University, No 1 Chang-Te Street, 10048, Taipei, Taiwan;Tissue Regeneration Product Technology Division, Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, 310, Hsinchu County, Taiwan;

关键词: Peri-implant osteomyelitis;    Magnetic nanoparticle;    Hyperthermia;    Biofilm;   

DOI  :  10.1186/s12879-017-2621-4

 received in 2017-05-03, accepted in 2017-07-19,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWhen bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis.MethodsSixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed.ResultsThe pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 °C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control.ConclusionsFor the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced.

【 授权许可】

CC BY   
© The Author(s). 2017