Transcription factor co-localization patterns affect human cell type-specific gene expression

BMC Genomics

Research Article

Augusto Rendon¹  Lorenz Wernisch¹  Willem Ouwehand¹  Dennis Wang² 

[1] Department of Haematology, University of Cambridge, Long Road, Cambridge, UK;MRC Biostatistics Unit, Institute of Public Health, Robinson Way, Cambridge, UK;

关键词: Transcriptional regulation;    Gene expression;    ChIP-Seq;    Regression modeling;   

DOI  :  10.1186/1471-2164-13-263

 received in 2012-03-16, accepted in 2012-06-12,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundCellular development requires the precise control of gene expression states. Transcription factors are involved in this regulatory process through their combinatorial binding with DNA. Information about transcription factor binding sites can help determine which combinations of factors work together to regulate a gene, but it is unclear how far the binding data from one cell type can inform about regulation in other cell types.ResultsBy integrating data on co-localized transcription factor binding sites in the K562 cell line with expression data across 38 distinct hematopoietic cell types, we developed regression models to describe the relationship between the expression of target genes and the transcription factors that co-localize nearby. With K562 binding sites identifying the predictors, the proportion of expression explained by the models is statistically significant only for monocytic cells (p-value< 0.001), which are closely related to K562. That is, cell type specific binding patterns are crucial for choosing the correct transcription factors for the model. Comparison of predictors obtained from binding sites in the GM12878 cell line with those from K562 shows that the amount of difference between binding patterns is directly related to the quality of the prediction. By identifying individual genes whose expression is predicted accurately by the binding sites, we are able to link transcription factors FOS, TAF1 and YY1 to a sparsely studied gene LRIG2. We also find that the activity of a transcription factor may be different depending on the cell type and the identity of other co-localized factors.ConclusionOur approach shows that gene expression can be explained by a modest number of co-localized transcription factors, however, information on cell-type specific binding is crucial for understanding combinatorial gene regulation.

【 授权许可】

CC BY   
© Wang et al.; licensee BioMed Central Ltd. 2012