Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery

BMC Plant Biology

Research Article

Xue-Qing Yan¹  Bai-Chen Wang²  Li-Jun Wang³  Wei Duan³  Hong-Guo Xu³  Shao-Hua Li⁴  Ling Ma⁵  Ji-Hu Li⁵  Bo-Fang Yan⁵  Guo-Tian Liu⁵ 

[1] Beijing Computing Center, 100094, Beijing, P. R. China;Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, P. R., China;Key laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, P. R., China;Key laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, P. R., China;Key laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botany Garden, Chinese Academy of Sciences, 430074, Wuhan, P. R., China;Key laboratory of Plant Resources and Beijing Key Laboratory of Grape Science and Enology, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, P. R., China;University of China Academy of Sciences, 100049, Beijing, P. R., China;

关键词: Cabernet sauvignon;    Heat stress;    iTRAQ;    Photosynthesis;    Proteomics;    Recovery;   

DOI  :  10.1186/1471-2229-14-110

 received in 2014-02-18, accepted in 2014-04-17,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHigh temperature is a major environmental factor limiting grape yield and affecting berry quality. Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of the thermotolerance of Vitis, we combined a physiological analysis with iTRAQ-based proteomics of Vitis vinifera cv Cabernet Sauvignon, subjected to 43°C for 6 h, and then followed by recovery at 25/18°C.ResultsHigh temperature increased the concentrations of TBARS and inhibited electronic transport in photosynthesis apparatus, indicating that grape leaves were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. One hundred and seventy-four proteins were differentially expressed under heat stress and/or during the recovery phase, in comparison to unstressed controls, respectively. Stress and recovery conditions shared 42 proteins, while 113 and 103 proteins were respectively identified under heat stress and recovery conditions alone. Based on MapMan ontology, functional categories for these dysregulated proteins included mainly photosynthesis (about 20%), proteins (13%), and stress (8%). The subcellular localization using TargetP showed most proteins were located in the chloroplasts (34%), secretory pathways (8%) and mitochondrion (3%).ConclusionOn the basis of these findings, we proposed that some proteins related to electron transport chain of photosynthesis, antioxidant enzymes, HSPs and other stress response proteins, and glycolysis may play key roles in enhancing grapevine adaptation to and recovery capacity from heat stress. These results provide a better understanding of the proteins involved in, and mechanisms of thermotolerance in grapevines.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.