Pulmonary challenge with carbon nanoparticles induces a dose-dependent increase in circulating leukocytes in healthy males

BMC Pulmonary Medicine

Research Article

Michiel Makkee¹  Johannes D. de Boer²  Elles M. Kemper³  Marieke Berger⁴  Peter J. Sterk⁴  René Lutter⁵  Jaring S. van der Zee⁶ 

[1] Catalysis Engineering, Chemical Engineering, Technical University of Delft, Delft, The Netherlands;Center for Experimental and Molecular Medicine (CEMM), Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands;Department of Pharmacy, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands;Department of Respiratory Medicine, Academic Medical Center, University of Amsterdam, Room F-5-260, Amsterdam, The Netherlands;Department of Respiratory Medicine, Academic Medical Center, University of Amsterdam, Room F-5-260, Amsterdam, The Netherlands;Department of Experimental Immunology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands;Department of Respiratory Medicine, Academic Medical Center, University of Amsterdam, Room F-5-260, Amsterdam, The Netherlands;Department of Respiratory Medicine, Onze Lieve Vrouwe Gasthuis, Amsterdam, The Netherlands;

关键词: Air pollution;    Coagulation;    Bronchial provocation test;    Bronchoalveolar lavage;    Ultrafine particles;    Inflammation;   

DOI  :  10.1186/s12890-017-0463-x

 received in 2016-10-24, accepted in 2017-08-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundInhalation of particulate matter, as part of air pollution, is associated with increased morbidity and mortality. Nanoparticles (< 100 nm) are likely candidates for triggering inflammatory responses and activation of coagulation pathways because of their ability to enter lung cells and pass bronchial mucosa. We tested the hypothesis that bronchial segmental instillation of carbon nanoparticles causes inflammation and activation of coagulation pathways in healthy humans in vivo.MethodsThis was an investigator-initiated, randomized controlled, dose-escalation study in 26 healthy males. Participants received saline (control) in one lung segment and saline (placebo) or carbon nanoparticles 10 μg, 50 μg, or 100 μg in the contra-lateral lung. Six hours later, blood and bronchoalveolar lavage fluid (BALF) was collected for inflammation and coagulation parameters.ResultsThere was a significant dose-dependent increase in blood neutrophils (p = 0.046) after challenge with carbon nanoparticles. The individual top-dose of 100 μg showed a significant (p = 0.05) increase in terms of percentage neutrophils in blood as compared to placebo.ConclusionsThis study shows a dose-dependent effect of bronchial segmental challenge with carbon nanoparticles on circulating neutrophils of healthy volunteers. This suggests that nanoparticles in the respiratory tract induce systemic inflammation.Trial registrationDutch Trial Register no. 2976. 11 July 2011. http://www.trialregister.nl/trialreg/admin/rctview.asp?TC=2976

【 授权许可】

CC BY   
© The Author(s). 2017