Transcriptome changes in grapevine (Vitis viniferaL.) cv. Malbec leaves induced by ultraviolet-B radiation

BMC Plant Biology

Research Article

Jose M Martinez-Zapater¹  Patricia N Piccoli²  Ruben Bottini²  Diego Lijavetzky³  Mariela A Pontin⁴  Rita Francisco⁵ 

[1] Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), C/Darwin 3, 28049, Madrid, España;Instituto de Ciencias de la Vid y del Vino (Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja), CCT, Campus de la Universidad de La Rioja, C/Madre de Dios 51, 26006, Logroño, España;Instituto de Biología Agrícola de Mendoza, Facultad de Ciencias Agrarias, Consejo Nacional de Investigaciones Científicas y Tecnológicas-Universidad Nacional de Cuyo, Almirante Brown 500, M5528AHB, Chacras de Coria, Argentina;Instituto de Biología Agrícola de Mendoza, Facultad de Ciencias Agrarias, Consejo Nacional de Investigaciones Científicas y Tecnológicas-Universidad Nacional de Cuyo, Almirante Brown 500, M5528AHB, Chacras de Coria, Argentina;Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), C/Darwin 3, 28049, Madrid, España;Instituto de Biología Agrícola de Mendoza, Facultad de Ciencias Agrarias, Consejo Nacional de Investigaciones Científicas y Tecnológicas-Universidad Nacional de Cuyo, Almirante Brown 500, M5528AHB, Chacras de Coria, Argentina;Estación Experimental Agropecuaria La Consulta INTA, cc8 (5567) La Consulta, San Carlos, Mendoza, Argentina;Plant Molecular Ecophysiology Laboratory, Instituto de Tecnologia Química e Biológica, Oeiras, Portugal;

关键词: Resveratrol;    Functional Category;    Fluence Rate;    Phototropic Response;    MapMan Software;   

DOI  :  10.1186/1471-2229-10-224

 received in 2010-06-29, accepted in 2010-10-20,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundUltraviolet-B radiation (UV-B, 280-315 nm) is a natural component of sunlight, which has numerous regulatory effects on plant physiology. The nature of the response to UV-B is dependent on fluence rate, dose, duration and wavelength of the UV-B treatment. Some reports have analyzed the changes in gene expression caused by UV-B light on several plant species using microarray technology. However, there is no information on the transcriptome response triggered by UV-B in grapevine. In this paper we investigate the gene expression responses of leaves from in vitro cultured Vitis vinifera cv. Malbec plants subjected to the same dose of biologically effective UV-B radiation (4.75 kJ m-2 d-1) administered at two different fluence rates (16 h at ≅ 8.25 μW cm-2, 4 h at ≅ 33 μW cm-2) using a new custom made GrapeGen Affymetrix GeneChip®.ResultsThe number of genes modulated by high fluence rate UV-B doubled the number of genes modulated by low fluence UV-B. Their functional analyses revealed several functional categories commonly regulated by both UV-B treatments as well as categories more specifically modulated depending on UV-B fluence rate. General protective responses, namely the induction of pathways regulating synthesis of UV-B absorbing compounds such as the Phenylpropanoid pathway, the induction of different antioxidant defense systems and the activation of pathways commonly associated with pathogen defense and abiotic stress responses seem to play critical roles in grapevine responses against UV-B radiation. Furthermore, high fluence rate UV-B seemed to specifically modulate additional pathways and processes in order to protect grapevine plantlets against UV-B-induced oxidative stress, stop the cell cycle progression, and control protein degradation. On the other hand, low fluence rate UV-B regulated the expression of specific responses in the metabolism of auxin and abscisic acid as well as in the modification of cell walls that could be involved in UV-B acclimation-like processes.ConclusionOur results show the UV-B radiation effects on the leaf transcriptome of grapevine (Vitis vinifera cv. Malbec) plantlets. Functional categories commonly modulated under both UV-B treatments as well as transcripts specifically regulated in an UV-B-intensity dependent way were identified. While high fluence rate UV-B had regulatory effects mainly on defense or general multiple-stress responses pathways, low fluence rate UV-B promoted the expression of genes that could be involved in UV-B protection or the amelioration of the UV-B-induced damage. This study also provides an extensive list of genes regulating multiple metabolic pathways involved in the response of grapevine to UV-B that can be used for future researches.

【 授权许可】

Unknown   
© Pontin et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.