Detrimental effect of apoptosis of lymphocytes at an early time point of experimental abdominal sepsis

BMC Infectious Diseases

Research Article

Konstantinos Atmatzidis¹  Stefanos Atmatzidis¹  Ioannis M Koutelidakis¹  Grigorios Chatzimavroudis¹  Anastasia Antonopoulou²  Konstantinos Louis²  Aikaterini Pistiki²  Antigone Kotsaki²  Evangelos J Giamarellos-Bourboulis²  Athina Savva² 

[1] 2nd Department of Surgery, University of Thessaloniki, Medical School, Thessaloniki, Greece;4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece;

关键词: apoptosis;    abdominal sepsis;    survival;    lymphocytes;    antimicrobials;   

DOI  :  10.1186/1471-2334-11-321

 received in 2011-06-30, accepted in 2011-11-20,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundApoptosis of lymphocytes is considered a late sequelum in the sepsis cascade. The role of apoptosis of lymphocytes as a driver of final outcome was investigated.MethodsAbdominal sepsis was induced after cecal ligation and puncture (CLP) in 31 rabbits. Blood was sampled at serial time intervals and peripheral blood mononuclear cells (PBMCs) were isolated. Apoptosis of lymphocytes and monocytes was measured through flow cytometric analysis. PBMCs were stimulated with LPS and Pam3Cys for the release of tumor necrosis factor-alpha (TNFα). Tissue bacterial growth was quantitatively measured. In a second set of experiments, CLP was performed in another 40 rabbits; 20 received single intravenous infusions of ciprofloxacin and of metronidazole 4 hours after surgery.ResultsAnimals were divided into two groups based on the percentage of lymphocyte apoptosis at 4 hours after surgery; less than or equal to 32% and more than 32%. Survival of the former was shorter than the latter (p: 0.017). Tissue growth was similar between groups. Apoptosis of lymphocytes and of monocytes was lower in the former group over follow-up. Release of ΤNFα did not differ. The above findings on survival were repeated in the second set of experiments. Administration of antimicrobials prolonged survival of the former group (p: 0.039) but not of the latter group (pNS).ConclusionsLymphocyte apoptosis at an early time point of experimental peritonitis is a major driver for death. A lower percentage of apoptosis leads earlier to death. Antimicrobials were beneficial even at that disease state.

【 授权许可】

Unknown   
© Atmatzidis et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.