Isoprene emission by poplar is not important for the feeding behaviour of poplar leaf beetles

BMC Plant Biology

Research Article

Anna Müller¹  Andrea Polle¹  Joop JA Van Loon²  Hans M Smid²  Marcel Dicke²  Gerrit Gort³  Basem Kanawati⁴  Philippe Schmitt-Kopplin⁴  Jörg-Peter Schnitzler⁵  Maaria Rosenkranz⁵  Patrick Faubert⁶  Moritz Kaling⁷ 

[1] Büsgen Institute, Forest Botany and Tree Physiology, University of Göttingen, Büsgenweg 2, 37077, Göttingen, Germany;Laboratory of Entomology, Wageningen University, P.O. Box 8031, NL-6700 EH, Wageningen, Netherlands;Mathematical and Statistical Methods Group, Wageningen University, P.O. Box 100, 6700 AC, Wageningen, Netherlands;Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, D-85764, Neuherberg, Germany;Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany;Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany;Département des Sciences Fondamentales, Chaire en éco-conseil, Université du Québec à Chicoutimi, 555, boul. de l’Université, G7H 2B1, Chicoutimi, Qc, Canada;Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Ingolstädter Landstraße 1, 85764, Neuherberg, Germany;Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, D-85764, Neuherberg, Germany;

关键词: Chrysomela populi;    Volatile organic compounds;    VOC;    Isoprene;    Isoprenoids;    Terpene;    Electroantennography;    Beetle behaviour;    Populus;    Metabolomics;   

DOI  :  10.1186/s12870-015-0542-1

 received in 2015-04-16, accepted in 2015-06-05,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundChrysomela populi (poplar leaf beetle) is a common herbivore in poplar plantations whose infestation causes major economic losses. Because plant volatiles act as infochemicals, we tested whether isoprene, the main volatile organic compound (VOC) produced by poplars (Populus x canescens), affects the performance of C. populi employing isoprene emitting (IE) and transgenic isoprene non-emitting (NE) plants. Our hypothesis was that isoprene is sensed and affects beetle orientation or that the lack of isoprene affects plant VOC profiles and metabolome with consequences for C. populi feeding.ResultsElectroantennographic analysis revealed that C. populi can detect higher terpenes, but not isoprene. In accordance to the inability to detect isoprene, C. populi showed no clear preference for IE or NE poplar genotypes in the choice experiments, however, the beetles consumed a little bit less leaf mass and laid fewer eggs on NE poplar trees in field experiments. Slight differences in the profiles of volatile terpenoids between IE and NE genotypes were detected by gas chromatography - mass spectrometry. Non-targeted metabolomics analysis by Fourier Transform Ion Cyclotron Resonance Mass Spectrometer revealed genotype-, time- and herbivore feeding-dependent metabolic changes both in the infested and adjacent undamaged leaves under field conditions.ConclusionsWe show for the first time that C. populi is unable to sense isoprene. The detected minor differences in insect feeding in choice experiments and field bioassays may be related to the revealed changes in leaf volatile emission and metabolite composition between the IE and NE poplars. Overall our results indicate that lacking isoprene emission is of minor importance for C. populi herbivory under natural conditions, and that the lack of isoprene is not expected to change the economic losses in poplar plantations caused by C. populi infestation.

【 授权许可】

Unknown   
© Müller et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.