Insights into naturally minimised Streptomyces albus J1074 genome

BMC Genomics

Research Article

Mariia Rabyk¹  Bohdan Ostash¹  Victor Fedorenko¹  Andriy Luzhetskyy²  Nestor Zaburannyi³ 

[1] Department Faculty of Biology, Ivan Franko National University of Lviv, Hrushevskogo str. 4, Lviv79005, Ukraine;Helmholtz-Institute for Pharmaceutical Research Saarland, Saarland University Campus, Building C2.3, 66123, Saarbrücken, Germany;Helmholtz-Institute for Pharmaceutical Research Saarland, Saarland University Campus, Building C2.3, 66123, Saarbrücken, Germany;Department Faculty of Biology, Ivan Franko National University of Lviv, Hrushevskogo str. 4, Lviv79005, Ukraine;

关键词: Biosynthetic Gene Cluster;    Ectoin;    Heterologous Production;    Nonribosomal Peptide Synthetase;    Wall Teichoic Acid;   

DOI  :  10.1186/1471-2164-15-97

 received in 2013-06-18, accepted in 2014-02-01,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery.ResultsTo improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.ConclusionsS. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain’s potential for natural product synthesis.

【 授权许可】

Unknown   
© Zaburannyi et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.