Maternal nutrient restriction in mid-to-late gestation influences fetal mRNA expression in muscle tissues in beef cattle

BMC Genomics

Research Article

Stephen P. Miller¹  Francois Paradis²  Carolyn Fitzsimmons³  Brian W. McBride⁴  Katie M. Wood⁴  Kendall C. Swanson⁵ 

[1] Angus Genetic Inc., 64506, St. Joseph, MO, USA;Department of Agriculture, Food, and Nutritional Science, University of Alberta, 4-10 Agriculture-Forestry Centre, T6G 2P5, Edmonton, AB, Canada;Department of Agriculture, Food, and Nutritional Science, University of Alberta, 4-10 Agriculture-Forestry Centre, T6G 2P5, Edmonton, AB, Canada;Agriculture and Agri-Food Canada, T6G 2C8, Edmonton, AB, Canada;Department of Animal Biosciences, University of Guelph, N1G 2W1, Guelph, ON, Canada;Department of Animal Science, North Dakota State University, 58102, Fargo, ND, USA;

关键词: Beef cattle;    fetal programming;    nutrient restriction;    liver;    muscle;    IGF2;    methylation;    microRNA;   

DOI  :  10.1186/s12864-017-4051-5

 received in 2016-08-29, accepted in 2017-08-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundManipulating maternal nutrition during specific periods of gestation can result in re-programming of fetal and post-natal development. In this experiment we investigated how a feed restriction of 85% compared with 140% of total metabolizable energy requirements, fed to cows during mid-to-late gestation, influences phenotypic development of fetuses and mRNA expression of growth (Insulin-Like Growth Factor family and Insulin Receptor (INSR)), myogenic (Myogenic Differentiation 1 (MYOD1), Myogenin (MYOG), Myocyte Enhancer Factor 2A (MEF2A), Serum Response Factor (SRF)) and adipogenic (Peroxisome Proliferator Activated Receptor Gamma (PPARG)) genes in fetal longissimus dorsi (LD) and semitendinosus (ST) muscle. DNA methylation of imprinted genes, Insulin Like Growth Factor 2 (IGF2) and Insulin Like Growth Factor 2 Receptor (IGF2R), and micro RNA (miRNA) expression, were also examined as potential consequences of poor maternal nutrition, but also potential regulators of altered gene expression patterns.ResultsWhile the nutrient restriction impacted dam body weight, no differences were observed in phenotypic fetal measurements (weight, crown-rump length, or thorax circumference). Interestingly, LD and ST muscles responded differently to the differential pre-natal nutrient levels. While LD muscle of restricted fetal calves had greater mRNA abundances for Insulin Like Growth Factor 1 and its receptor (IGF1 and IGF1R), IGF2R, INSR, MYOD1, MYOG, and PPARG, no significant differences were observed for gene expression in ST muscle. Similarly, feed restriction had a greater impact on the methylation level of IGF2 Differentially Methylated Region 2 (DMR2) in LD muscle as compared to ST muscle between treatment groups. A negative correlation existed between IGF2 mRNA expression and IGF2 DMR2 methylation level in both LD and ST muscles. Differential expression of miRNAs 1 and 133a were also detected in LD muscle.ConclusionsOur data suggests that a nutrient restriction of 85% as compared to 140% of total metabolizable energy requirements during the 2nd half of gestation can alter the expression of growth, myogenic and adipogenic genes in fetal muscle without apparent differences in fetal phenotype. It also appears that the impact of feed restriction varies between muscles suggesting a priority for nutrient partitioning depending on muscle function and/or fiber composition. Differences in the methylation level in IGF2, a well-known imprinted gene, as well as differences in miRNA expression, may be functional mechanisms that precede the differences in gene expression observed, and could lead to trans-generational epigenetic programming.

【 授权许可】

CC BY   
© The Author(s). 2017