The genome of the emerging barley pathogen Ramularia collo-cygni

BMC Genomics

Research Article

Linda J. Paterson¹  Ashleigh Mackenzie¹  Fiona Burnett¹  Kalina Gorniak¹  Graham R. D. McGrann¹  Francois Dussart¹  Jeanette M. G. Taylor¹  Neil D. Havis¹  Elisabet Sjökvist²  Maciej Kaczmarek³  James M. Fountaine⁴  Ambrose Andongabo⁵  Kostya Kanyuka⁶  Kim E. Hammond-Kosack⁶  Jason J. Rudd⁶  Urmi Trivedi⁷  Mark Blaxter⁸ 

[1] Crop Protection Team, Crop and Soil Systems Group, SRUC, West Mains Road, EH9 3JG, Edinburgh, UK;Crop Protection Team, Crop and Soil Systems Group, SRUC, West Mains Road, EH9 3JG, Edinburgh, UK;Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, EH9 3TF, Edinburgh, UK;Crop Protection Team, Crop and Soil Systems Group, SRUC, West Mains Road, EH9 3JG, Edinburgh, UK;Present address: Forest Research, Alice Holt Lodge, GU10 4LH, Farnham, Surrey, UK;Crop Protection Team, Crop and Soil Systems Group, SRUC, West Mains Road, EH9 3JG, Edinburgh, UK;Present address: Syngenta, Jealott’s Hill International Research Centre, RG42 6EY, Bracknell, Berkshire, UK;Department of Computational and Systems Biology, Rothamsted Research, AL5 2JQ, Harpenden, Hertfordshire, UK;Department of Plant Biology and Crop Science, Rothamsted Research, AL5 2JQ, Harpenden, Hertfordshire, UK;Edinburgh Genomics, The University of Edinburgh, EH9 3JT, Edinburgh, UK;Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, EH9 3TF, Edinburgh, UK;Edinburgh Genomics, The University of Edinburgh, EH9 3JT, Edinburgh, UK;

关键词: Ramularia leaf spot;    Dothideomycetes;    Rubellin toxin;    Endophyte;    Necrotroph;    Whole genome sequencing;   

DOI  :  10.1186/s12864-016-2928-3

 received in 2015-11-09, accepted in 2016-07-12,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundRamularia collo-cygni is a newly important, foliar fungal pathogen of barley that causes the disease Ramularia leaf spot. The fungus exhibits a prolonged endophytic growth stage before switching life habit to become an aggressive, necrotrophic pathogen that causes significant losses to green leaf area and hence grain yield and quality.ResultsThe R. collo-cygni genome was sequenced using a combination of Illumina and Roche 454 technologies. The draft assembly of 30.3 Mb contained 11,617 predicted gene models. Our phylogenomic analysis confirmed the classification of this ascomycete fungus within the family Mycosphaerellaceae, order Capnodiales of the class Dothideomycetes. A predicted secretome comprising 1053 proteins included redox-related enzymes and carbohydrate-modifying enzymes and proteases. The relative paucity of plant cell wall degrading enzyme genes may be associated with the stealth pathogenesis characteristic of plant pathogens from the Mycosphaerellaceae. A large number of genes associated with secondary metabolite production, including homologs of toxin biosynthesis genes found in other Dothideomycete plant pathogens, were identified.ConclusionsThe genome sequence of R. collo-cygni provides a framework for understanding the genetic basis of pathogenesis in this important emerging pathogen. The reduced complement of carbohydrate-degrading enzyme genes is likely to reflect a strategy to avoid detection by host defences during its prolonged asymptomatic growth. Of particular interest will be the analysis of R. collo-cygni gene expression during interactions with the host barley, to understand what triggers this fungus to switch from being a benign endophyte to an aggressive necrotroph.

【 授权许可】

CC BY   
© The Author(s). 2016