Virtual reality assisted microscopy data visualization and colocalization analysis

BMC Bioinformatics

Research

Rensu P. Theart¹  Ben Loos¹  Thomas R. Niesler¹ 

[1] Stellenbosch University, 7600, Stellenbosch, South Africa;

关键词: Virtual reality;    Hand tracking;    Colocalization analysis;    Region of interest selection;    Confocal microscopy visualization;    3D Microscopic reconstruction;    Volume rendering;   

DOI  :  10.1186/s12859-016-1446-2

来源: Springer

【 摘 要 】

BackgroundConfocal microscopes deliver detailed three-dimensional data and are instrumental in biological analysis and research. Usually, this three-dimensional data is rendered as a projection onto a two-dimensional display. We describe a system for rendering such data using a modern virtual reality (VR) headset. Sample manipulation is possible by fully-immersive hand-tracking and also by means of a conventional gamepad. We apply this system to the specific task of colocalization analysis, an important analysis tool in biological microscopy. We evaluate our system by means of a set of user trials.ResultsThe user trials show that, despite inaccuracies which still plague the hand tracking, this is the most productive and intuitive interface. The inaccuracies nevertheless lead to a perception among users that productivity is low, resulting in a subjective preference for the gamepad. Fully-immersive manipulation was shown to be particularly effective when defining a region of interest (ROI) for colocalization analysis.ConclusionsVirtual reality offers an attractive and powerful means of visualization for microscopy data. Fully immersive interfaces using hand tracking show the highest levels of intuitiveness and consequent productivity. However, current inaccuracies in hand tracking performance still lead to a disproportionately critical user perception.

【 授权许可】

CC BY   
© The Author(s) 2017

【 图 表 】

12864_2016_2790_Article_IEq1.gif

12864_2016_3353_Article_IEq29.gif

12864_2015_2129_Article_IEq8.gif

12888_2016_877_Article_IEq20.gif

12888_2016_877_Article_IEq19.gif

12711_2017_365_Article_IEq33.gif