BMC Complementary and Alternative Medicine | |
Carbon tetrachloride induced kidney and lung tissue damages and antioxidant activities of the aqueous rhizome extract of Podophyllum hexandrum | |
Research Article | |
Mohmmad Afzal Zargar1  Zahid Mahmood1  Akbar Masood1  Showkat Ahmad Ganie1  Ehtishamul Haq2  Bilal Ahmad Zargar3  Yasrib Qurishi4  Abid Hamid4  | |
[1] Department of Biochemistry, University of Kashmir, 190006, Srinagar, India;Department of Biotechnology, University of Kashmir, 190006, Srinagar, India;Department of Pharmaceutical Sciences, University of Kashmir, 190006, Srinagar, India;Indian Institute of Integrative Medicine (Council of Scientific & Industrial Research), Jammu, India; | |
关键词: Glutathione Reductase; Aqueous Extract; Radical Scavenge Activity; Butylate Hydroxy Toluene; Super Oxide Dismutase Activity; | |
DOI : 10.1186/1472-6882-11-17 | |
received in 2010-11-08, accepted in 2011-02-28, 发布年份 2011 | |
来源: Springer | |
【 摘 要 】
BackgroundThe present study was conducted to evaluate the in vitro and in vivo antioxidant properties of aqueous extract of Podophyllum hexandrum. The antioxidant potential of the plant extract under in vitro situations was evaluated by using two separate methods, inhibition of superoxide radical and hydrogen peroxide radical. Carbon tetrachloride (CCl4) is a well known toxicant and exposure to this chemical is known to induce oxidative stress and causes tissue damage by the formation of free radicals.Methods36 albino rats were divided into six groups of 6 animals each, all animals were allowed food and water ad libitum. Group I (control) was given olive oil, while the rest groups were injected intraperitoneally with a single dose of CCl4 (1 ml/kg) as a 50% (v/v) solution in olive oil. Group II received CCl4 only. Group III animals received vitamin E at a concentration of 50 mg/kg body weight and animals of groups IV, V and VI were given extract of Podophyllum hexandrum at concentration dose of 20, 30 and 50 mg/kg body weight. Antioxidant status in both kidney and lung tissues were estimated by determining the activities of antioxidative enzymes, glutathione reductase (GR), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and superoxide dismutase (SOD); as well as by determining the levels of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS). In addition, superoxide and hydrogen peroxide radical scavenging activity of the extract was also determined.ResultsResults showed that the extract possessed strong superoxide and hydrogen peroxide radical scavenging activity comparable to that of known antioxidant butylated hydroxy toluene (BHT). Our results also showed that CCl4 caused a marked increase in TBARS levels whereas GSH, SOD, GR, GPX and GST levels were decreased in kidney and lung tissue homogenates of CCl4 treated rats. Aqueous extract of Podophyllum hexandrum successfully prevented the alterations of these effects in the experimental animals.ConclusionOur study demonstrated that the aqueous extract of Podophyllum hexandrum could protect the kidney and lung tissue against CCl4 induced oxidative stress probably by increasing antioxidant defense activities.
【 授权许可】
Unknown
© Ganie et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
【 预 览 】
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