Presence of intestinal Mycobacterium avium subspecies paratuberculosis(MAP) DNA is not associated with altered MMP expression in ulcerative colitis

BMC Gastroenterology

Research Article

Martin Roderfeld¹  Annika Rhode¹  Elke Roeb¹  Sonja Blöcher¹  Timo Rath¹  Jörg M Halwe²  Tina Basler³  Ralph Goethe³  Michael Bülte⁴  Amir Abdulmawjood⁴  Ömer Akineden⁴ 

[1] Department of Gastroenterology, Medical Clinic II, Justus Liebig University, Paul-Meimberg-Strasse 5, 35392, Giessen, Germany;Department of Internal Medicine, University Hospital Stavanger, 4011, Stavanger, Norway;Department of Internal Medicine, Stord Hospital, 5416, Stord, Norway;Institute for Microbiology, Centre for Infectious Medicine, Veterinarian Medical Academy Hannover, Bischofsholer Damm 15, 30173, Hannover, Germany;Institute of Veterinary Food Science, Faculty of Veterinary Medicine, Justus Liebig University, Frankfurter Strasse 92, 35392, Giessen, Germany;

关键词: Inflammatory Bowel Disease;    Inflammatory Bowel Disease Patient;    Ulcerative Colitis Patient;    Mycobacterium Avium Subspecies Paratuberculosis;    Internal Amplification Control;   

DOI  :  10.1186/1471-230X-11-34

 received in 2010-10-26, accepted in 2011-04-08,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundMycobacterium avium subspecies paratuberculosis (MAP) is suspected to be a causative agent in human Crohn's disease (CD). Recent evidence suggests that pathogenic mycobacteria and MAP can induce the expression of Matrix Metalloproteinases (MMP), which are the main proteases in the pathogenesis of mucosal ulcerations in inflammatory bowel disease (IBD). Within this study we assessed the prevalence of intestinal MAP specific DNA in patients with Crohn's disease, ulcerative colitis (UC), and healthy controls. We further analysed regulation patterns of MMPs in mucosal tissues of UC patients with and without intestinal MAP DNA detection.MethodsColonic biopsy samples were obtained from 63 Norwegian and German IBD patients and 21 healthy controls. RNA was quantified by quantitative real-time polymerase chain reaction (PCR) to study MMP gene expression in both pathological and healthy mucosal specimens. The presence of MAP DNA in colonic mucosa was examined using MAP specific PCR.ResultsMAP DNA was detected in 20% of UC patients and 33% of healthy controls but only in 7% of patients with CD. UC patients treated with corticosteroids exhibited a significantly increased frequency of intestinal MAP DNA compared to those not receiving corticosteroids. Expression of MMP-1, -2, -7, -9, -13, -19, -28 and TNF-α did not differ between UC patients with presence of intestinal MAP DNA compared to those without. MMP-2, MMP-9 and MMP-13 were significantly decreased in UC patients receiving corticosteroids.ConclusionsThe presence of intestinal MAP specific DNA is not associated with altered MMP expression in UC in vivo. Corticosteroids are associated with increased detection of intestinal MAP DNA and decreased expression of certain MMPs. Frequent detection of MAP DNA in healthy controls might be attributable to the wide environmental distribution of MAP and its presence in the food-chain.

【 授权许可】

Unknown   
© Rath et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.