An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil

BMC Genomics

Research Article

Paulo FP Pimenta¹  José MC Ribeiro²  Eric Calvo²  Andrezza C Chagas³ 

[1] Entomology Laboratory, Centro de Pesquisa René Rachou, Belo Horizonte, Minas Gerais, Brazil;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, 20892-8132, Rockville, Maryland, USA;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, 20892-8132, Rockville, Maryland, USA;Entomology Laboratory, Centro de Pesquisa René Rachou, Belo Horizonte, Minas Gerais, Brazil;

关键词: Tryptic Peptide;    Blood Feeding;    Salivary Protein;    Onchocerciasis;    Strong Bootstrap Support;   

DOI  :  10.1186/1471-2164-12-612

 received in 2011-07-15, accepted in 2011-12-19,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLittle is known about the composition and function of the saliva in black flies such as Simulium guianense, the main vector of river blindness disease in Brazil. The complex salivary potion of hematophagous arthropods counteracts their host's hemostasis, inflammation, and immunity.ResultsTranscriptome analysis revealed ubiquitous salivary protein families--such as the Antigen-5, Yellow, Kunitz domain, and serine proteases--in the S. guianense sialotranscriptome. Insect-specific families were also found. About 63.4% of all secreted products revealed protein families found only in Simulium. Additionally, we found a novel peptide similar to kunitoxin with a structure distantly related to serine protease inhibitors. This study revealed a relative increase of transcripts of the SVEP protein family when compared with Simulium vittatum and S. nigrimanum sialotranscriptomes. We were able to extract coding sequences from 164 proteins associated with blood and sugar feeding, the majority of which were confirmed by proteome analysis.ConclusionsOur results contribute to understanding the role of Simulium saliva in transmission of Onchocerca volvulus and evolution of salivary proteins in black flies. It also consists of a platform for mining novel anti-hemostatic compounds, vaccine candidates against filariasis, and immuno-epidemiologic markers of vector exposure.

【 授权许可】

Unknown   
© Chagas et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.