Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

BMC Biotechnology

Research Article

Dede Abdulrachman¹  Antonius Suwanto¹  Lily Eurwilaichitr²  Thidarat Nimchua²  Duriya Chantasingh²  Kanokarn Kocharin²  Verawat Champreda²  Paweena Thongkred²  Monthon Nakpathom³  Buppha Somboon³  Nootsara Narumol³ 

[1] Faculty of Biotechnology, Atmajaya Catholic University, Jl. Jend. Sudirman 51, 12930, Jakarta, Indonesia;Microbial Biotechnology and Biochemicals Research Unit, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Pahonyothin Rd, 12120, Khlong Luang, Patumthani, Thailand;Textile Laboratory, Polymers Research Unit, National Metal and Materials Technology Center, 114 Thailand Science Park, Pahonyothin Rd, 12120, Khlong Luang, Patumthani, Thailand;

关键词: Acidic pectinase;    Bioscouring;    Cotton fabric;    Heterologous expression;    High cell density fermentation;   

DOI  :  10.1186/s12896-017-0334-9

 received in 2016-04-30, accepted in 2017-02-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundRemoval of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation.ResultsThe recombinant EndoPG exhibited a specific activity of 1892.08 U/mg on citrus pectin under the optimal condition at 50 °C, pH 5.0 with a Vmax and Km of 65,451.35 μmol/min/mL and 15.14 mg/mL, respectively. A maximal activity of 2408.70 ± 26.50 U/mL in the culture supernatant was obtained by high cell density batch fermentation, equivalent to a 4.8 times greater yield than that from shake-flask culture. The recombinant enzyme was shown to be suitable for application as a bioscouring agent, in which the wettability of cotton fabric was increased by treatment with enzyme at 300 U/mL scouring solution at 40 °C, pH 5.0 for 1 h. The bio-scoured fabric has comparable wettability to that obtained by conventional chemical scouring, but has higher tensile strength.ConclusionThe work has demonstrated for the first time functions of A. aculeatus EndoPG on bioscouring in eco-textile processing. EndoPG alone was shown to possess effective scouring activity. High expression level and homogeneity could be achieved in bench-scale bioreactor.

【 授权许可】

CC BY   
© The Author(s). 2017