Chemosensory gene expression in olfactory organs of the anthropophilic Anopheles coluzzii and zoophilic Anopheles quadriannulatus

BMC Genomics

Research Article

L. V. Cosme¹  M. A. Slotman²  Z. Popkin-Hall²  S. Pathikonda²  G. Athrey³  W. Takken⁴ 

[1] Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, USA;Department of Entomology, Texas A&M University, 77845, College Station, TX, USA;Department of Poultry Science, Texas A&M University, College Station, TX, USA;Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands;

关键词: Olfaction;    Chemosensory genes;    Host seeking;    Malaria vector;    RNAseq;    Antennae;    Maxillary palp;   

DOI  :  10.1186/s12864-017-4122-7

 received in 2017-04-13, accepted in 2017-09-06,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundAnopheles (An.) coluzzii, one of Africa’s primary malaria vectors, is highly anthropophilic. This human host preference contributes greatly to its ability to transmit malaria. In contrast, the closely related An. quadriannulatus prefers to feed on bovids and is not thought to contribute to malaria transmission. The diverged preference for host odor profiles between these sibling species is likely reflected in chemosensory gene expression levels in the olfactory organs. Therefore, we compared the transcriptomes of the antennae and maxillary palps between An. coluzzii and An. quadriannulatus, focusing on the major chemosensory gene families.ResultsWhile chemosensory gene expression is strongly correlated between the two species, various chemosensory genes show significantly enhanced expression in one of the species. In the antennae of An. coluzzii the expression of six olfactory receptors (Ors) and seven ionotropic receptors (Irs) is considerably enhanced, whereas 11 Ors and 3 Irs are upregulated in An. quadriannulatus. In the maxillary palps, leaving aside Irs with very low level of expression, one Ir is strongly enhanced in each species. In addition, we find divergence in odorant binding protein (Obp) gene expression, with several highly expressed Obps being enhanced in the antennae and palps of An. coluzzii. Finally, the expression of several gustatory receptors (Grs) in the palps appears to be species-specific, including a homolog of a sugar-sensing Drosophila Gr.ConclusionsA considerable number of Ors and Irs are differentially expressed between these two closely related species with diverging host preference. These chemosensory genes could play a role in the human host preference of the malaria vector An. coluzzii. Additionally, divergence in Obp expression between the two species suggests a possible role of these odor carrier proteins in determining host preference. Finally, divergence in chemosensory expression in the palps may point towards a possible role for the maxillary palps in host differentiation.

【 授权许可】

CC BY   
© The Author(s). 2017