Altered expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder by 17β-estradiol

BMC Urology

Research Article

Kyung Aa Cho¹  Seung-Chul Lee²  Jong Sung Park³  Seung Hee Song⁴  Dongdeuk Kwon⁴  Kwangsung Park⁴  Sun-Ouck Kim⁴ 

[1] Department of Biochemistry, Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea;Department of Dermatology, Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea;Department of Physiology, Research Institute of Medical Sciences, Chonnam National University, Gwangju, South Korea;Department of Urology, Research Institute of Medical Sciences, Chonnam National University, 8, Hak-dong, 501-757, Dong-ku, Gwangju, South Korea;

关键词: Caveolin;    Bladder;    Estrogen;    Rat;   

DOI  :  10.1186/1471-2490-13-44

 received in 2013-05-20, accepted in 2013-09-10,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe purpose of this study was to investigate the effect of estrogen alteration on the expression of caveolin 2 and 3 in rat smooth muscle of urinary bladder.MethodsFemale Sprague–Dawley rats were divided into three groups: control, bilateral ovariectomy (Ovx), and bilateral ovariectomy followed by subcutaneous injections of 17β-estradiol (Ovx?+?Est). After 4 weeks, urodynamic measurements were taken to ascertain the contraction interval and contraction pressure. The expression and cellular localization of caveolin 2 and 3 were determined by Western blot and immunohistochemistry in rat urinary bladder smooth muscle.ResultsIn cystometrograms, the contraction interval (min) was significantly lower in the Ovx group (3.1?±?1.5) than in the control group (5.6?±?1.2), but was increased after estrogen treatment (9.3?±?1.0). Conversely, the average contraction pressure (mmHg) was higher in the Ovx group (26.2?±?2.3) than in the control group (21.9?±?3.1), and was decreased after estrogen treatment (23.8?±?3.5). Caveolin 2 and 3 expression was localized in the cell membrane of the smooth muscle. The protein expression of both caveolin 2 and 3 was significantly lower after ovariectomy and was restored to the control levels after 17β-estradiol treatment.ConclusionsHormonal alteration causes a significant change in the expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder. These findings suggest that these molecules might have functional roles in the detrusor overactivity that occurs in association with hormonal alteration.

【 授权许可】

Unknown   
© Kim et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.