Toxicological evaluation of Terminalia paniculata bark extract and its protective effect against CCl4-induced liver injury in rodents

BMC Complementary and Alternative Medicine

Research Article

Hitesh V Jagani¹  Anoop Kishore²  Nitesh Kumar²  Rekha R Shenoy²  Punit Bansal²  Pawan G Nayak²  Sahil Talwar²  Krishnadas Nandakumar² 

[1] Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, 576 104, Manipal, Karnataka, India;Department of Pharmacology, Manipal College of Pharmaceutical Sciences,Manipal University, 576 104, Manipal, Karnataka, India;

关键词: Terminalia paniculata;    Antioxidant;    Hepatoprotective;    CCl-induced liver toxicity;    Mitochondrial staining;    Apoptotic markers;   

DOI  :  10.1186/1472-6882-13-127

 received in 2013-01-16, accepted in 2013-05-28,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundBased on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage.MethodsIntrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and sub-chronic toxicity studies in mice. TPW was then evaluated against CCl4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCl4-treated Chang cells were also elucidated.ResultsTPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCl4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCl4-treated Chang cells, improving the viability considerably.ConclusionThe findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.

【 授权许可】

Unknown   
© Talwar et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.