期刊论文详细信息
BMC Complementary and Alternative Medicine
Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells
Research Article
Farid Saleh1  Raj Raghupathy2  Sami Asfar3  Noha Al-Saleh4  Medhat Oteifa4 
[1] Department of Anatomy, College of Medicine, Al-Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia;Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait;Department of Surgery, Faculty of Medicine, Kuwait University, Kuwait City, Kuwait;Department of Surgery, Kuwait Cancer Control Centre, Kuwait City, Kuwait;
关键词: EGCG;    PBMC;    IFN-γ;    Green tea;   
DOI  :  10.1186/1472-6882-14-322
 received in 2013-09-09, accepted in 2014-08-27,  发布年份 2014
来源: Springer
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【 摘 要 】

BackgroundCancer immunotherapy requires proper manipulation of the immune system, lymphocytes in particular, in order to identify and destroy the cancer cells as non-self. In this study we investigated the effect of the flavonoid present in green tea, namely epigallocatechin-3-gallate (EGCG), on the proliferation of, and IFN-γ production by, peripheral blood mononuclear cells (PBMC) from breast cancer patients stimulated with a mitogen, anti-CD3 and the common breast cancer peptides Her-2/neu, and p53.MethodsBlood samples were collected from 25 patients with breast cancer at the Kuwait Cancer Control Centre (KCCC). The patients were newly diagnosed, and had not undergone any treatment or surgery at the time of sample collection. The control group consisted of 25 healthy women age-matched (±5 years) to the patients. PBMC were isolated from the patients and controls, and were cultured separately with the mitogen PHA, anti-CD3 antibodies, and Her-2/neu and p53 in the presence or absence of standardized doses of EGCG. The degree of proliferation and interferon-γ [IFN-γ) release were then analyzed.ResultsEGCG significantly suppressed the proliferation of PBMC in response to stimulation separately with (i) the mitogen, (ii) anti-CD3, and (iii) the cancer antigen peptides. IFN-γ production was also significantly suppressed by EGCG in vitro.ConclusionsEGCG appears to have an immunosuppressive effect on the proliferation of PBMC, indicating that EGCG is worth exploring for immunomodulatory effects in autoimmune diseases and tissue transplantation.

【 授权许可】

CC BY   
© Saleh et al.; licensee BioMed Central Ltd. 2014

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