Chitosan gel vaccine protects against tumour growth in an intracaecal mouse model of cancer by modulating systemic immune responses

BMC Immunology

Research Article

Roslyn A. Kemp¹  Georgia M. Bell¹  Andrew J. Highton¹  Adam Girardin¹  Sarah M. Hook² 

[1]Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, PO Box 56, 9054, Dunedin, New Zealand

[2]School of Pharmacy, University of Otago, Dunedin, New Zealand

关键词: Colon cancer;    IFN-γ;    T cells;    Vaccination;    Tumour;    Caecum;   

DOI  :  10.1186/s12865-016-0178-4

 received in 2016-07-02, accepted in 2016-10-13,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundVaccination generating a robust memory population of CD8+ T cells may provide protection against cancer. However, immune therapies for cancer are influenced by the local tumour immune microenvironment. An infiltrate of T cells into tumours of people with colorectal cancer has proven to be a significant indicator of good prognosis.MethodsWe used an intracaecal mouse model of cancer to determine whether a protective immune response against a mucosal gut tumour could be generated using a systemic intervention. We investigated the generation of murine memory CD8+ T cells using a sustained antigen release vaccine vehicle (chitosan gel; Gel + OVA) containing the model antigen ovalbumin, chitosan gel alone (Gel) or conventional dendritic cell vaccination (DC + OVA) using the same protein antigen.ResultsFollowing vaccination with Gel + OVA, CD8+ T cell memory populations specific for ovalbumin protein were detected. Only vaccination with Gel + OVA gave decreased tumour burden compared to unvaccinated or DC + OVA-vaccinated mice in the intracaecal cancer challenge model.ConclusionThese results indicate that subcutaneous vaccination with Gel + OVA generates a population of functional CD8+ memory T cells in lymphoid tissue able to protect against intracaecal tumour challenge. Vaccination with chitosan gel may be valuable in anti-cancer treatment at both peripheral and mucosal sites.

【 授权许可】

CC BY   
© The Author(s). 2016